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Survivin反义寡核苷酸对人卵巢癌细胞株IL-6／STAT3信号通路的影响和意义
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Journal Article
Survivin反义寡核苷酸对人卵巢癌细胞株IL-6／STAT3信号通路的影响和意义
2017
Overview
摘要：目的通过分析人卵巢癌细胞株转染Survivin反义寡核苷酸（As0DN）后IL-6／STAT3信号通路及其下游相关癌基因的表达以及其侵袭能力的改变，探讨Survivin ASODN在抑制人卵巢癌细胞转移中的可能作用、机制和临床价值。方法脂质体（Lipofectamine 2000）介导SurvivinASODN转染人卵巢癌SKOV3细胞株（实验组），同时设空脂质体对照，Transwell小室检测细胞株侵袭能力的改变，荧光定量PCR检测两组细胞株中白细胞介素6（IL-6）、信号转导与活化因子3（STAT3）、Survivin、血管内皮生长因子（VEGF）基因的表达，Westernblot检测IL-6、STAT3、磷酸化的信号转导与活化因子3（p-STAT3）、Survivin、VEGF-A蛋白的表达。结果实验组细胞株中IL-6、STAT3、Survivin、VEGF基因表达均较对照组明显下调（P〈0．05）；实验组细胞株中IE-6、STAT3、p-STAT3、Survivin、vEGF-A蛋白表达均较对照组明显下调（P〈0．05）。实验组细胞株侵袭能力较对照组明显下降（P〈0．01）。结论ASODN靶向抑制Survivin可有效降低人卵巢癌细胞侵袭转移能力，抑制IL-6／STAT3信号通路及其下游相关致癌基因的表达活性。Survivin ASODN可能在防治卵巢癌复发和转移治疗中有临床价值。
Publisher
河北医科大学 第二医院妇产科，河北石家庄 050000,河北医科大学 第三医院妇产科，河北石家庄 050051%河北医科大学 第二医院妇产科,河北石家庄,050000
Subject
