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核转录因子激活蛋白-1对PPARδ表达的调控作用
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Journal Article
核转录因子激活蛋白-1对PPARδ表达的调控作用
2005
Overview
目的研究在过氧化物酶体增长因子活化受体δ（PPARδ）表达中的相关调控因子。方法用RT-PCR扩增人类PPARδ启动子序列，通过Deletion及重组将不同长短的PPARδ启动子序列转化到PGL3-basic载体中，转染LOVO细胞，通过观测各重组体转染活性，确定PPARδ启动子活性区域。再通过电泳迁移率变更分析（EMSA）、突变等方法确定核转录因子激活蛋白-1（AP1）对PPARδ表达的作用。结果Deletion及重组转染后发现PPARδ启动子活性区域在序列3361～3464之间，EMSA、突变发现AP1因子结合位点突变后转染活性明显降低。结论AP1因子结合位点突变后转染活性明显降低，提示AP1是PPARδ的一个增强子。
Publisher
西安交通大学环境与疾病相关基因教育部重点实验室,陕西西安,710061%北京大学医学部生化系,北京,853006,西安交通大学医学院遗传与分子生物学系
Subject
