Asset Details
Do you wish to reserve the book?
人骨髓间充质干细胞定向血管内皮细胞诱导分化前后eNOS表达/活性及其代谢产物的差异
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
人骨髓间充质干细胞定向血管内皮细胞诱导分化前后eNOS表达/活性及其代谢产物的差异
Do you wish to request the book?
人骨髓间充质干细胞定向血管内皮细胞诱导分化前后eNOS表达/活性及其代谢产物的差异
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
人骨髓间充质干细胞定向血管内皮细胞诱导分化前后eNOS表达/活性及其代谢产物的差异
2017
Overview
目的探讨人骨髓间充质干细胞（hBMSCs）定向血管内皮细胞诱导分化前后内皮型一氧化氮合酶（eNOS）基因表达、活性及其代谢产物的差异。方法建立hBMSCs定向血管内皮细胞诱导分化系统。应用倒置显微镜观察细胞形态的改变,Transwell实验检测细胞的迁移能力,细胞免疫荧光及Western blot检测eNOS的蛋白表达,ELISA法检测eNOS的活性,硝酸还原法检测细胞培养上清液NO含量。结果与未分化组相比,分化后的细胞形态发生明显改变;细胞的迁移能力增强238.10%（73.000±7.002 vs.21.000±4.359,P〈0.05）;eNOS蛋白的表达增加114.72%（0.423±0.011 vs.0.197±0.079,P〈0.05）;eNOS的活性增强157.49%（4.967±0.073 vs.1.929±0.103,P〈0.05）;NO合成与释放增加155.67%（184.909±1.853 vs.72.323±0.426,P〈0.05）。结论 hBMSCs定向内皮细胞诱导分化后eNOS基因表达增加、活性增强,其代谢产物NO的合成与释放增加。本结果可能为干细胞在心血管疾病等防治提供依据。
Publisher
广西医科大学药学院,广西南宁,530021
Subject
We currently cannot retrieve any items related to this title. Kindly check back at a later time.