Asset Details
Do you wish to reserve the book?
绿原酸对视网膜母细胞瘤细胞株HXO-RB44的生长抑制作用
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
绿原酸对视网膜母细胞瘤细胞株HXO-RB44的生长抑制作用
Do you wish to request the book?
绿原酸对视网膜母细胞瘤细胞株HXO-RB44的生长抑制作用
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
绿原酸对视网膜母细胞瘤细胞株HXO-RB44的生长抑制作用
2016
Overview
目的探讨绿原酸（CHA）对体外培养人视网膜母细胞瘤（Rb）细胞株HXO-RB44的作用及机制。方法应用CCK-8体外抑制实验,筛选CHA对体外培养传6代的人Rb细胞株HXO-RB44的最佳抑癌浓度。Western blot检测人Rb细胞株HXO-RB44中抑癌基因Rb1和P16及细胞周期蛋白（Cyclin D2）和细胞周期蛋白依赖激酶（CDK 4）的表达变化;ELISA检测培养基中乳酸脱氢酶（LDH）水平。结果 CCK-8体外抑制实验结果显示,CHA对人Rb细胞株HXO-RB44的最佳抑癌浓度为80μmol/L。经80μmol/L CHA处理5d的HXO-RB44细胞株中,抑癌基因Rb1和P16均有少量表达,而在阳性对照组（HT1080细胞）和未处理的HXO-RB44细胞几乎没有表达。CHA处理5d的HXO-RB44细胞株的Cyclin D2和CDK4的表达明显低于阳性对照组（HT1080细胞）和未处理的HXO-RB44细胞（P〈0.01）。CHA处理5d的HXO-RB44的培养基中LDH的含量为（226.75±42.74）U/L,明显低于正常培养的细胞培养基（425.84±31.67）U/L（P〈0.01）。结论 80μmol/L CHA可能通过增加HXO-RB44抑癌基因的表达,降低细胞周期蛋白的表达及LDH水平,而抑制HXO-RB44细胞株的增殖。
Publisher
辽宁抚顺市眼病医院,辽宁抚顺113008%辽河油田总医院眼科,辽宁盘锦,124010%锦州医科大学病理生理学教研室,辽宁锦州,121001%锦州医科大学解剖学教研室,辽宁锦州,121001,锦州医科大学解剖学教研室,辽宁锦州 121001
Subject
