Asset Details
Do you wish to reserve the book?
Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia
Do you wish to request the book?
Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification and Genetic Characterization of Blastocystis Species in Patients from Makkah, Saudi Arabia
2022
Overview
species (sp.) are gastrointestinal protozoan parasites with high prevalence rates worldwide.
sp. show extensive genetic diversity with 17 different subtypes (STs) described to date. A few studies have investigated the prevalence and STs of
sp. in Makkah, Saudi Arabia. Therefore, we aimed in this study to identify and characterize subtypes of
sp. in the City of Makkah, Saudi Arabia.
Stool samples were collected from 140 patients who presented to King Abdulaziz Hospital, Hera General Hospital and Modern Medical Center in Saudi Arabia. Different microscopic examination methods of patients' stools and molecular analyses (using primers targeting SSU rRNA gene) were performed to identify and characterize STs of
sp.
Our microscopic examination of stool samples showed that 96/140 patients (68.6%) had
sp. infection. Clinical examination of infected patients revealed that 81 patients were symptomatic, whereas 15 were asymptomatic. Next, we isolated DNA from
sp.-positive stool samples followed by PCR amplification of small-subunit ribosomal RNA (SSU rRNA) gene and sequence analysis. Our sequence analysis showed that subtype 3 (ST3) was the most prevalent (53.13%) followed by subtype 1 (ST1) (45.83%), whereas subtype 2 (ST2) was the least prevalent (1.04%). Moreover, our results showed that all three STs resulted in more symptomatic than asymptomatic cases. Finally, we identified novel haplotypes which comprised of 8 ST3, 6 ST1, and one ST2 haplotypes.
Our identification of several haplotypes in patients' stools confirms the genetic diversity of
sp. and may explain the reported low host specificity and differential pathogenicity of
sp. We believe that additional molecular epidemiological and genomic studies are needed to understand the prevalence and pathogenicity of different subtypes in humans and animal hosts.
