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aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用
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Journal Article
aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用
2017
Overview
目的观察aptamer-siRNA核酸复合物对人慢性粒细胞白血病（CML）细胞株K562细胞凋亡的影响,并探讨其作用机制。方法使用不同浓度的aptamer-siRNA溶液转染K562细胞,噻唑蓝（MTT）法检测aptamer-siRNA对K562细胞增殖的影响,AnnexinV/PI双染法检测aptamer-siRNA对K562细胞凋亡的影响,Western blot和RT-PCR法检测aptamer-siRNA对K562细胞bcl-2、Bax和caspase-3蛋白及mRNA表达的影响。结果与对照组相比,转染aptamer-siRNA后K562细胞增殖受到明显抑制,K562细胞的早期凋亡率明显增加,细胞中bcl-2蛋白和mRNA的表达水平明显降低,Bax和caspase-3蛋白和mRNA的表达水平明显升高,差异均具有统计学意义（P〈0.05）。aptamersiRNA核酸复合物浓度（50~250μmol/L）对bcl-2、Bax和caspase-3 mRNA的影响具有明显的量效关系。结论aptamer-siRNA核酸复合物能够通过促使bcl-2基因减少和Bax、caspase-3基因增长,进而促进K562细胞凋亡。
Publisher
石家庄市第五医院检验科,河北石家庄,050021%昆明医科大学海源学院细胞生物学暨医学遗传学教研室,云南昆明,650106
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