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结肠癌患者外周血DcR3与CEA联合测定的临床价值
by
胜利 安利峰 史霖 王军阳 范桂香 袁育康
in
DcR3
/ 癌胚抗原
/ 结肠癌
2007
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结肠癌患者外周血DcR3与CEA联合测定的临床价值
by
胜利 安利峰 史霖 王军阳 范桂香 袁育康
in
DcR3
/ 癌胚抗原
/ 结肠癌
2007
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Journal Article
结肠癌患者外周血DcR3与CEA联合测定的临床价值
2007
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目的 联合检测结肠癌患者外周血中DcR3与癌胚抗原(CEA)mRNA的水平,建立取材于外周血的早期诊断结肠癌的新方法。方法 44例结肠癌患者和30名健康志愿者,各取5mL静脉血,肝素抗凝,密度梯度离心法分离外周血单核细胞,细胞总RNA TRIzol提取液提取外周血单核细胞总RNA,用半定量RTPCR法检测外周血单核细胞中DcR3与CEA mRNA的水平,并用t检验进行比较。结果 结肠癌患者外周血中DcR3基因的相对表达水平为0.415±0.078,与健康对照组(0.235±0.074)比较有显著性差异(P〈0.05),但患者之间没有明显差异(P〉0.05)。CEA在结肠癌患者外周血中阳性率为36.4%(16/44),表达水平为0.346±0.071。30名健康志愿者均为阴性。结论 DcR3基因在结肠癌患者外周血中表达水平明显高于健康人,其表达水平与结肠癌有相关性。在CEA阴性情况下,DcR3可能成为新的肿瘤标志物。
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