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适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术
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适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术
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Journal Article
适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术
2015
Overview
目的建立适用于细粒棘球蚴囊液蛋白质组的双向电泳分离条件。方法冷冻干燥法制备囊液总蛋白提取物,观察不同的蛋白样品处理方法、IPG胶条pH范围和电泳上样量对双向电泳图谱的影响。结果经ReadyPrepTM2-D Cleanup Kit处理的提取物上样量为400μg,在pH7-10范围的胶条进行双向电泳分离,可获取蛋白斑点较多、重复性较好的二维电泳图谱。结论建立的细粒棘球蚴囊液双向电泳技术及图谱,可为细粒棘球蚴虫蛋白质组学的研究提供理论依据。
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