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PIMGAVir and Vir-MinION: Two Viral Metagenomic Pipelines for Complete Baseline Analysis of 2nd and 3rd Generation Data
by
Mastriani, Emilio
, Wong, Gary
, Bienes, Kathrina Mae
, Berthet, Nicolas
in
2nd and 3rd sequencing generation
/ Analysis
/ Classification
/ computer software
/ Data mining
/ DNA sequencing
/ Epidemics
/ Evaluation
/ Genomes
/ Infectious diseases
/ Internet
/ Life Sciences
/ metagenomic pipeline
/ Metagenomics
/ multiple strategies analysis
/ nanopores
/ Nucleotide sequencing
/ Pathogens
/ Quality control
/ scientists
/ taxonomic classification
/ Taxonomy
/ Technical Note
/ Zoonoses
2022
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PIMGAVir and Vir-MinION: Two Viral Metagenomic Pipelines for Complete Baseline Analysis of 2nd and 3rd Generation Data
by
Mastriani, Emilio
, Wong, Gary
, Bienes, Kathrina Mae
, Berthet, Nicolas
in
2nd and 3rd sequencing generation
/ Analysis
/ Classification
/ computer software
/ Data mining
/ DNA sequencing
/ Epidemics
/ Evaluation
/ Genomes
/ Infectious diseases
/ Internet
/ Life Sciences
/ metagenomic pipeline
/ Metagenomics
/ multiple strategies analysis
/ nanopores
/ Nucleotide sequencing
/ Pathogens
/ Quality control
/ scientists
/ taxonomic classification
/ Taxonomy
/ Technical Note
/ Zoonoses
2022
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PIMGAVir and Vir-MinION: Two Viral Metagenomic Pipelines for Complete Baseline Analysis of 2nd and 3rd Generation Data
by
Mastriani, Emilio
, Wong, Gary
, Bienes, Kathrina Mae
, Berthet, Nicolas
in
2nd and 3rd sequencing generation
/ Analysis
/ Classification
/ computer software
/ Data mining
/ DNA sequencing
/ Epidemics
/ Evaluation
/ Genomes
/ Infectious diseases
/ Internet
/ Life Sciences
/ metagenomic pipeline
/ Metagenomics
/ multiple strategies analysis
/ nanopores
/ Nucleotide sequencing
/ Pathogens
/ Quality control
/ scientists
/ taxonomic classification
/ Taxonomy
/ Technical Note
/ Zoonoses
2022
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PIMGAVir and Vir-MinION: Two Viral Metagenomic Pipelines for Complete Baseline Analysis of 2nd and 3rd Generation Data
Journal Article
PIMGAVir and Vir-MinION: Two Viral Metagenomic Pipelines for Complete Baseline Analysis of 2nd and 3rd Generation Data
2022
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Overview
The taxonomic classification of viral sequences is frequently used for the rapid identification of pathogens, which is a key point for when a viral outbreak occurs. Both Oxford Nanopore Technologies (ONT) MinION and the Illumina (NGS) technology provide efficient methods to detect viral pathogens. Despite the availability of many strategies and software, matching them can be a very tedious and time-consuming task. As a result, we developed PIMGAVir and Vir-MinION, two metagenomics pipelines that automatically provide the user with a complete baseline analysis. The PIMGAVir and Vir-MinION pipelines work on 2nd and 3rd generation data, respectively, and provide the user with a taxonomic classification of the reads through three strategies: assembly-based, read-based, and clustering-based. The pipelines supply the scientist with comprehensive results in graphical and textual format for future analyses. Finally, the pipelines equip the user with a stand-alone platform with dedicated and various viral databases, which is a requirement for working in field conditions without internet connection.
Publisher
MDPI AG,MDPI
Subject
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