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神经生长因子对糖皮质激素诱导大鼠海马神经元凋亡的保护作用
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神经生长因子对糖皮质激素诱导大鼠海马神经元凋亡的保护作用
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Journal Article
神经生长因子对糖皮质激素诱导大鼠海马神经元凋亡的保护作用
2017
Overview
目的探讨神经生长因子对糖皮质激素诱导的大鼠海马神经元凋亡的保护作用。方法体外分离原代培养18只新生Wister大鼠海马神经元,噻唑蓝法测定地塞米松诱导海马神经元凋亡的最低敏感剂量,观察不同质量浓度神经生长因子对地塞米松（0.10×10^-6mol/L）诱导海马神经元凋亡的保护作用。结果与阴性对照组相比,地塞米松Ⅰ组（10×10^-6mol/L）、Ⅱ组（1×10^-6mol/L）和Ⅲ组（0.10×10^-6mol/L）大鼠海马神经元活性均降低（P=0.000,0.000,0.000）。予不同质量浓度神经生长因子后,神经生长因子0.18 ng/ml组大鼠海马神经元活性低于阴性对照组（P=0.000）和阳性对照组（P=0.010）,神经生长因子18 ng/ml组大鼠海马神经元活性高于阳性对照组（P=0.000）和神经生长因子0.18 ng/ml组（P=0.000）。结论糖皮质激素可以诱导体外培养的大鼠海马神经元凋亡,地塞米松0.10×10^-6mol/L是诱导海马神经元凋亡的最低敏感剂量,神经生长因子可以拮抗地塞米松诱导的大鼠海马神经元凋亡。
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