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miRNA-29a在神经细胞的表达特征
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Journal Article
miRNA-29a在神经细胞的表达特征
2015
Overview
目的观察微小RNA（mi RNA）-29a在小鼠神经系统内的表达特征。方法实时定量聚合酶链反应检测经原代培养第3、7、15、21天星形胶质细胞、神经元和出生后相应时间点小鼠大脑皮质mi RNA-29a表达变化。结果随着小鼠生长发育,mi RNA-29a在神经系统内的表达逐渐增强,与体外原代培养第3天相比,第7、15、21天时神经元和星形胶质细胞mi RNA-29a相对表达量均升高（P〈0.05或P〈0.01）;与饲养第3天小鼠相比,饲养第7、15、21天小鼠大脑皮质mi RNA-29a相对表达量亦升高（P〈0.05或P〈0.01）;同一观察时间点星形胶质细胞mi RNA-29a相对表达量是神经元的20~40倍（P=0.000）,而大脑皮质mi RNA-29a相对表达量仅为星形胶质细胞的1/3（P=0.000）。结论 mi RNA-29a在小鼠大脑皮质发育过程中呈现的高丰度特异性表达,主要表现为星形胶质细胞mi RNA-29a表达上调。
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