Asset Details
Do you wish to reserve the book?
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
Do you wish to request the book?
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
2013
Overview
The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca ²⁺ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca ²⁺ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca ²⁺ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activity-based competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca ²⁺ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Animals, Genetically Modified
/ Axons
/ Biological and medical sciences
/ Calcium
/ Embryos
/ Fundamental and applied biological sciences. Psychology
/ Humans
/ Neural Pathways - embryology
/ Neural Pathways - physiology
/ Neurons
/ Plasmids
/ Potassium Channels, Inwardly Rectifying - genetics
/ Potassium Channels, Inwardly Rectifying - metabolism
/ Proteins
/ Receptors, Cell Surface - antagonists & inhibitors
/ Receptors, Cell Surface - genetics
/ Receptors, Cell Surface - physiology
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Vertebrates: nervous system and sense organs
/ Zebrafish Proteins - antagonists & inhibitors
