Asset Details
Do you wish to reserve the book?
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions
Do you wish to request the book?
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions
2011
Overview
Prokaryotes have evolved multiple versions of an RNA-guided adaptive immune system that targets foreign nucleic acids. In each case, transcripts derived from clustered regularly interspaced short palindromic repeats (CRISPRs) are thought to selectively target invading phage and plasmids in a sequence-specific process involving a variable cassette of CRISPR-associated (cas) genes. The CRISPR locus in Pseudomonas aeruginosa (PA14) includes four cas genes that are unique to and conserved in microorganisms harboring the Csy-type (CRISPR system yersinia) immune system. Here we show that the Csy proteins (Csy1-4) assemble into a 350 kDa ribonucleoprotein complex that facilitates target recognition by enhancing sequence-specific hybridization between the CRISPR RNA and complementary target sequences. Target recognition is enthalpically driven and localized to a \"seed sequence\" at the 5' end of the CRISPR RNA spacer. Structural analysis of the complex by small-angle X-ray scattering and single particle electron microscopy reveals a crescent-shaped particle that bears striking resemblance to the architecture of a large CRISPR-associated complex from Escherichia coli, termed Cascade. Although similarity between these two complexes is not evident at the sequence level, their unequal subunit stoichiometry and quaternary architecture reveal conserved structural features that may be common among diverse CRISPR-mediated defense systems.
Publisher
National Academy of Sciences,National Academy of Sciences Issue,National Acad Sciences
Subject
/ Adaptive Immunity - genetics
/ Adaptive Immunity - immunology
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ DNA
/ E coli
/ Endoribonucleases - genetics
/ Endoribonucleases - metabolism
/ Escherichia coli - immunology
/ Genes
/ loci
/ Macromolecular Substances - chemistry
/ Macromolecular Substances - metabolism
/ Phages
/ Plasmids
/ Proteins
/ Pseudomonas aeruginosa - genetics
/ Pseudomonas aeruginosa - immunology
/ RNA
/ Seeds
/ Spacer
/ Yersinia
