Journal Article

The Effects of Cadmium Exposure on the Oxidative State and Cell Death in the Gill of Freshwater Crab Sinopotamon henanense

Wang, Jinxiang, Wang, Lan, Liu, Dongmei, Wang, Qian, Shen, Qingqing, Li, Jing, Zhang, Pingping

2013

Overview

We studied here the short-term toxicity effects of Cd on the oxidative state and cell death in the gill of freshwater crab Sinopotamon henanense. Crabs were exposed to Cd that resulted in Cd accumulation and a significant increase in the metallothionein (MT) level in the gill, but MT level increased disproportionally compared to the Cd accumulation with an extension of exposure time. Significant changes in the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were observed. An increase in the levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) was detected that will cause oxidative stress. Histological abnormalities of the gills were discovered, including the expansion of gill cavity, a decrease in the numbers of connection of the upper and the lower of the gill lamellae and epithelial cells, and an increase in the number of hemocytes. The results of a TUNEL test and transmission electron microscope (TEM) showed that more gill cells had apoptotic characteristics after 48 h of Cd treatment compared to the control, but epithelial cell necrosis and inflammatory response appeared only after 72 h. It was concluded that (1) Cd induced the ROS production and accumulation through inhibiting antioxidant enzyme activities and exceeding the saturation values of MT binging; (2) Cd led to lipid peroxidation and histopathological alternations; and (3) Cd induced apoptotic response at short time exposure, followed by necrotic features and inflammatory reaction after longer time exposure.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abnormalities / Accumulation / Alternations / Animals / Antioxidants / Antioxidants - metabolism / Apoptosis / Apoptosis - drug effects / Biology / Cadmium / Cadmium - analysis / Cadmium - toxicity / Catalase / Cell death / Chemistry / Crabs / Crustaceans / Cytotoxicity / Decapoda (Crustacea) - cytology / Decapoda (Crustacea) - drug effects / Decapoda (Crustacea) - metabolism / Earth Sciences / Enzymatic activity / Epithelial cells / Epithelium / Exposure / Gangrene / Gene expression / Gills / Gills - cytology / Gills - drug effects / Gills - metabolism / Gills - pathology / Glutathione / Glutathione peroxidase / Hemocytes / Inflammation / Inflammatory response / Lamellae / Life sciences / Lipid peroxidation / Lipids / Metallothionein / Metallothionein - metabolism / Necrosis / Necrosis - chemically induced / Oncorhynchus mykiss / Oxidative stress / Oxidative Stress - drug effects / Oxygen / Peroxidase / Peroxidation / Reactive oxygen species / Rodents / Sinopotamon / Superoxide dismutase / Time Factors / Toxicity / Toxicity Tests, Acute / Water - chemistry