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MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
by
Zhong, Ruiqin
, Ye, Zheng-Hua
, Richardson, Elizabeth A
in
Arabidopsis
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Arabidopsis thaliana
/ Base Sequence
/ biochemical pathways
/ Biosynthesis
/ Cell Nucleus
/ Cell Nucleus - metabolism
/ Cell Wall
/ Cell Wall - metabolism
/ Cell Wall - ultrastructure
/ cell walls
/ Cellulose
/ chromatin
/ Chromatin Immunoprecipitation
/ cytology
/ DNA
/ electrophoresis
/ Electrophoretic Mobility Shift Assay
/ Fiber cells
/ Fibers
/ Gene expression regulation
/ Gene Expression Regulation, Plant
/ gene overexpression
/ Genes
/ genetics
/ Lignin
/ metabolism
/ Molecular Sequence Data
/ Plant Epidermis
/ Plant Epidermis - cytology
/ Plant Epidermis - metabolism
/ Plant Leaves
/ Plant Leaves - cytology
/ Plant Leaves - metabolism
/ Plant Stems
/ Plant Stems - genetics
/ Plants
/ program planning
/ Promoter Regions, Genetic
/ Protein Binding
/ Protein Transport
/ Reporter genes
/ Repression
/ Repressor Proteins
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Sequence Homology, Amino Acid
/ stems
/ Trans-Activators
/ Trans-Activators - metabolism
/ transcription (genetics)
/ Transcription factors
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ transcriptional activation
/ ultrastructure
/ Up-Regulation
/ xylan
/ Xylem
/ Xylem - ultrastructure
2007
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MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
by
Zhong, Ruiqin
, Ye, Zheng-Hua
, Richardson, Elizabeth A
in
Arabidopsis
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Arabidopsis thaliana
/ Base Sequence
/ biochemical pathways
/ Biosynthesis
/ Cell Nucleus
/ Cell Nucleus - metabolism
/ Cell Wall
/ Cell Wall - metabolism
/ Cell Wall - ultrastructure
/ cell walls
/ Cellulose
/ chromatin
/ Chromatin Immunoprecipitation
/ cytology
/ DNA
/ electrophoresis
/ Electrophoretic Mobility Shift Assay
/ Fiber cells
/ Fibers
/ Gene expression regulation
/ Gene Expression Regulation, Plant
/ gene overexpression
/ Genes
/ genetics
/ Lignin
/ metabolism
/ Molecular Sequence Data
/ Plant Epidermis
/ Plant Epidermis - cytology
/ Plant Epidermis - metabolism
/ Plant Leaves
/ Plant Leaves - cytology
/ Plant Leaves - metabolism
/ Plant Stems
/ Plant Stems - genetics
/ Plants
/ program planning
/ Promoter Regions, Genetic
/ Protein Binding
/ Protein Transport
/ Reporter genes
/ Repression
/ Repressor Proteins
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Sequence Homology, Amino Acid
/ stems
/ Trans-Activators
/ Trans-Activators - metabolism
/ transcription (genetics)
/ Transcription factors
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ transcriptional activation
/ ultrastructure
/ Up-Regulation
/ xylan
/ Xylem
/ Xylem - ultrastructure
2007
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MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
by
Zhong, Ruiqin
, Ye, Zheng-Hua
, Richardson, Elizabeth A
in
Arabidopsis
/ Arabidopsis - genetics
/ Arabidopsis - metabolism
/ Arabidopsis Proteins
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Arabidopsis thaliana
/ Base Sequence
/ biochemical pathways
/ Biosynthesis
/ Cell Nucleus
/ Cell Nucleus - metabolism
/ Cell Wall
/ Cell Wall - metabolism
/ Cell Wall - ultrastructure
/ cell walls
/ Cellulose
/ chromatin
/ Chromatin Immunoprecipitation
/ cytology
/ DNA
/ electrophoresis
/ Electrophoretic Mobility Shift Assay
/ Fiber cells
/ Fibers
/ Gene expression regulation
/ Gene Expression Regulation, Plant
/ gene overexpression
/ Genes
/ genetics
/ Lignin
/ metabolism
/ Molecular Sequence Data
/ Plant Epidermis
/ Plant Epidermis - cytology
/ Plant Epidermis - metabolism
/ Plant Leaves
/ Plant Leaves - cytology
/ Plant Leaves - metabolism
/ Plant Stems
/ Plant Stems - genetics
/ Plants
/ program planning
/ Promoter Regions, Genetic
/ Protein Binding
/ Protein Transport
/ Reporter genes
/ Repression
/ Repressor Proteins
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Sequence Homology, Amino Acid
/ stems
/ Trans-Activators
/ Trans-Activators - metabolism
/ transcription (genetics)
/ Transcription factors
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ transcriptional activation
/ ultrastructure
/ Up-Regulation
/ xylan
/ Xylem
/ Xylem - ultrastructure
2007
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MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
Journal Article
MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
2007
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Overview
We demonstrate that the Arabidopsis thaliana MYB46 transcription factor is a direct target of SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1), which is a key transcriptional activator regulating the developmental program of secondary wall biosynthesis. The MYB46 gene is expressed predominantly in fibers and vessels in stems, and its encoded protein is targeted to the nucleus and can activate transcription. MYB46 gene expression was shown to be regulated by SND1, and transactivation analysis demonstrated that SND1 as well as its close homologs were able to activate the MYB46 promoter. Electrophoretic mobility shift assays and chromatin immunoprecipitation experiments revealed that SND1 binds to the MYB46 promoter. Dominant repression of MYB46 caused a drastic reduction in the secondary wall thickening of fibers and vessels. Overexpression of MYB46 resulted in an activation of the biosynthetic pathways of cellulose, xylan, and lignin and concomitantly led to ectopic deposition of secondary walls in cells that are normally nonsclerenchymatous. In addition, the expression of two secondary wall-associated transcription factors, MYB85 and KNAT7, was highly upregulated by MYB46 overexpression. These results demonstrate that MYB46 is a direct target of SND1 and is another key player in the transcriptional network involved in the regulation of secondary wall biosynthesis in ARABIDOPSIS:
Publisher
American Society of Plant Biologists
Subject
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Chromatin Immunoprecipitation
/ cytology
/ DNA
/ Electrophoretic Mobility Shift Assay
/ Fibers
/ Gene Expression Regulation, Plant
/ Genes
/ genetics
/ Lignin
/ Plant Epidermis - metabolism
/ Plants
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Sequence Homology, Amino Acid
/ stems
/ Trans-Activators - metabolism
/ Transcription Factors - genetics
/ Transcription Factors - metabolism
/ xylan
/ Xylem
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