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chemostat array enables the spatio-temporal analysis of the yeast proteome
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chemostat array enables the spatio-temporal analysis of the yeast proteome
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Journal Article
chemostat array enables the spatio-temporal analysis of the yeast proteome
2013
Overview
Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP strains in five additional conditions for a total of more than 10,000 unique experiments, providing a systematic view of the yeast proteome in flux. We observed that processing bodies formed rapidly and synchronously in response to UV irradiation, and in conjunction with 506 deletion-GFP strains, identified four gene disruptions leading to abnormal ribonucleotide-diphosphate reductase (Rnr4) localization. Our microchemostat platform enables the large-scale interrogation of proteomes in flux and permits the concurrent observation of protein abundance, localization, cell size, and growth parameters on the single-cell level for thousands of microbial cultures in one experiment.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Cells
/ Genes
/ Green Fluorescent Proteins - metabolism
/ Imaging
/ Methyl Methanesulfonate - pharmacology
/ Microfluidics - instrumentation
/ proteome
/ Saccharomyces cerevisiae - drug effects
/ Saccharomyces cerevisiae - growth & development
/ Saccharomyces cerevisiae - metabolism
/ Saccharomyces cerevisiae Proteins - metabolism
/ Yeast
/ Yeasts
