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The Dynamics of Genomic-Length DNA Molecules in 100-nm Channels
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The Dynamics of Genomic-Length DNA Molecules in 100-nm Channels
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Journal Article
The Dynamics of Genomic-Length DNA Molecules in 100-nm Channels
2004
Overview
We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of λ DNA multimers with a 130-nm SD in 1 min.
Publisher
National Academy of Sciences,National Acad Sciences
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