Journal Article

Profiling Dynamic Patterns of Single‐Cell Motility

Jayatilaka, Hasini, Walston, Jeremy, Maity, Debonil, Sivakumar, Nikita, Cerchietti, Leandro, Phillip, Jude M., Min, Chanhong, Kamat, Pratik, Zamponi, Nahuel, Melnick, Ari, Venturutti, Leandro, Du, Wenxuan, Agrawal, Anshika, Johnston, Adrian, Starich, Bartholomew, Riley, Deanna

2024

Overview

Cell motility plays an essential role in many biological processes as cells move and interact within their local microenvironments. Current methods for quantifying cell motility typically involve tracking individual cells over time, but the results are often presented as averaged values across cell populations. While informative, these ensemble approaches have limitations in assessing cellular heterogeneity and identifying generalizable patterns of single‐cell behaviors, at baseline and in response to perturbations. In this study, CaMI is introduced, a computational framework designed to leverage the single‐cell nature of motility data. CaMI identifies and classifies distinct spatio‐temporal behaviors of individual cells, enabling robust classification of single‐cell motility patterns in a large dataset (n = 74 253 cells). This framework allows quantification of spatial and temporal heterogeneities, determination of single‐cell motility behaviors across various biological conditions and provides a visualization scheme for direct interpretation of dynamic cell behaviors. Importantly, CaMI reveals insights that conventional cell motility analyses may overlook, showcasing its utility in uncovering robust biological insights. Together, a multivariate framework is presented to classify emergent patterns of single‐cell motility, emphasizing the critical role of cellular heterogeneity in shaping cell behaviors across populations. Authors present CaMI, a novel computational framework designed to leverage single‐cell motility to deduce novel biological insights. CaMI identifies distinct spatio‐temporal behaviors of cells to enable robust classification of cell motility patterns. Furthermore, CaMI provides a means to directly compute cellular heterogeneity, quantify single‐cell behaviors across multiple conditions, and a visualization scheme for direct interpretation of emergent cell behaviors.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
• Subject: cell motility / Cell Movement - physiology / Cells / Datasets / high‐throughput cell phenotyping / Humans / Motility / Single-Cell Analysis - methods / single‐cell behaviors