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Pathways and mechanisms for efficient nitrogen utilization in the high-temperature tolerant heterotrophic nitrifying bacterium Aeribacillus pallidus sp. GW-E
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Pathways and mechanisms for efficient nitrogen utilization in the high-temperature tolerant heterotrophic nitrifying bacterium Aeribacillus pallidus sp. GW-E
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Journal Article
Pathways and mechanisms for efficient nitrogen utilization in the high-temperature tolerant heterotrophic nitrifying bacterium Aeribacillus pallidus sp. GW-E
2025
Overview
This study isolated a novel thermophilic heterotrophic nitrifying bacterium,
Aeribacillus pallidus
sp. GW-E, from aerobic composting. Under conditions of 55 °C, the utilization efficiency of NH₄⁺-N, NO₃⁻-N, and NO₂⁻-N were 87.42%, 21.44%, and 51.68%, respectively. Whole-genome analysis identified key nitrogen metabolism genes (
amt
,
npd
,
nirA
,
gdhA
,
glnA
,
and gltBD
) as well as heat stress-related genes (
GRPE
,
hslO
,
groES
,
groEL
). Response surface optimization revealed that under conditions of a C/N ratio of 15, a temperature of 54 °C, and a pH of 8, the NH₄⁺-N utilization efficiency reached 100%. Enzyme activity assays indicated that the activities of three enzymes in the ammonia assimilation pathway were GS 1.014 ± 0.030 U/mg, GDH 1.114 ± 0.090 U/mg, and GOGAT 11.611 ± 0.061 U/mg, which were significantly higher than those of other pathways (
P
< 0.05). Nitrogen balance analysis confirmed that approximately 40.04% of the nitrogen was assimilated. In conclusion, the bacterium primarily utilizes ammonia assimilation, with additional assimilated nitrate reduction and nitrification pathways for nitrogen transformation. This strain represents a valuable microbial resource and provides a theoretical basis for nitrogen retention in high-temperature composting systems.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Bacillaceae - classification
/ Bacillaceae - isolation & purification
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ Control
/ Identification and classification
/ Metabolic Networks and Pathways - genetics
/ Methods
/ Mycology
/ Nitrogen transformation pathways
/ Virology
