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Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori
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Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori
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Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori
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Journal Article
Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori
2009
Overview
Background
Through the whole life of eukaryotes, autophagy plays an important role in various biological events including development, differentiation and determination of lifespan. A full set of genes and their encoded proteins of this evolutionarily conserved pathway have been identified in many eukaryotic organisms from yeast to mammals. However, this pathway in the insect model organism, the silkworm
Bombyx mori
, remains poorly investigated.
Results
Based on the autophagy pathway in several model organisms and a series of bioinformatic analyses, we have found more than 20 autophagy-related genes from the current database of the silkworm
Bombyx mori
. These genes could be further classified into the signal transduction pathway and two ubiquitin-like pathways. Using the mRNA extracted from the silkgland, we cloned the full length cDNA fragments of some key genes via reverse transcription PCR and 3' rapid amplification of cDNA ends (RACE). In addition, we found that the transcription levels of two indicator genes
BmATG8
and
BmATG12
in the silkgland tend to be increased from 1
st
to 8
th
day of the fifth instar larvae.
Conclusion
Bioinformatics in combination with RT-PCR enable us to remodel a preliminary pathway of autophagy in the silkworm. Amplification and cloning of most autophagy-related genes from the silkgland indicated autophagy is indeed an activated process. Furthermore, the time-course transcriptional profiles of
BmATG8
and
BmATG12
revealed that both genes are up-regulated along the maturation of the silkgland during the fifth instar. These findings suggest that the autophagy should play an important role in
Bombyx mori
silkgland.
Publisher
BioMed Central,BioMed Central Ltd
Subject
