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High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana
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High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana
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High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana
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Journal Article
High light stress triggers distinct proteomic responses in the marine diatom Thalassiosira pseudonana
2016
Overview
Background
Diatoms are able to acclimate to frequent and large light fluctuations in the surface ocean waters. However, the molecular mechanisms underlying these acclimation responses of diaotms remain elusive.
Results
In this study, we investigated the mechanism of high light protection in marine diatom
Thalassiosira pseudonana
using comparative proteomics in combination with biochemical analyses. Cells treated under high light (800 μmol photons m
−2
s
−1
) for 10 h were subjected to proteomic analysis. We observed that 143 proteins were differentially expressed under high light treatment. Light-harvesting complex proteins, ROS scavenging systems, photorespiration, lipid metabolism and some specific proteins might be involved in light protection and acclimation of diatoms. Non-photochemical quenching (NPQ) and relative electron transport rate could respond rapidly to varying light intensities. High-light treatment also resulted in increased diadinoxanthin + diatoxanthin content, decreased Fv/Fm, increased triacylglycerol and altered fatty acid composition. Under HL stress, levels of C14:0 and C16:0 increased while C20:5ω3 decreased.
Conclusions
We demonstrate that
T. pseudonana
has efficient photoprotective mechanisms to deal with HL stress.
De novo
synthesis of Ddx/Dtx and lipid accumulation contribute to utilization of the excess energy. Our data will provide new clues for in-depth study of photoprotective mechanisms in diatoms.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
