Asset Details
Do you wish to reserve the book?
Intracellular and extracellular forces drive primary cilia movement
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Intracellular and extracellular forces drive primary cilia movement
Do you wish to request the book?
Intracellular and extracellular forces drive primary cilia movement
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Intracellular and extracellular forces drive primary cilia movement
2015
Overview
Primary cilia are ubiquitous, microtubule-based organelles that play diverse roles in sensory transduction in many eukaryotic cells. They interrogate the cellular environment through chemosensing, osmosensing, and mechanosensing using receptors and ion channels in the ciliary membrane. Little is known about the mechanical and structural properties of the cilium and how these properties contribute to ciliary perception. We probed the mechanical responses of primary cilia from kidney epithelial cells [Madin–Darby canine kidney-II (MDCK-II)], which sense fluid flow in renal ducts. We found that, on manipulation with an optical trap, cilia deflect by bending along their length and pivoting around an effective hinge located below the basal body. The calculated bending rigidity indicates weak microtubule doublet coupling. Primary cilia of MDCK cells lack interdoublet dynein motors. Nevertheless, we found that the organelles display active motility. 3D tracking showed correlated fluctuations of the cilium and basal body. These angular movements seemed random but were dependent on ATP and cytoplasmic myosin-II in the cell cortex. We conclude that force generation by the actin cytoskeleton surrounding the basal body results in active ciliary movement. We speculate that actin-driven ciliary movement might tune and calibrate ciliary sensory functions.
Significance A single primary cilium extends from the surface of many mammalian cells—often into an aqueous lumen, such as a kidney duct. In kidney epithelial cells, primary cilia are believed to sense fluid flow. This mechanosensory function is critical for proper organ function. Fluid flow is assumed to deflect cilia, leading to activation of transmembrane ion channels. This study defines the mechanical contributions of both bending and pivoting at the base to ciliary deflection. In addition, we report that active intracellular forces drive ciliary pivoting. This cell-directed cilia movement may be important for tuning ciliary mechanosensitivity.
