Journal Article

Effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (Solanum tuberosum)

Azevedo, João Lúcio, Andreote, Fernando Dini, Araújo, Welington Luiz, Rocha, Ulisses Nunes da, van Overbeek, Leonard Simon

2010

Overview

Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization. The major factors contributing to these interactions are not always well understood for most bacterial and plant species. It is believed that specific bacterial functions are required for plant colonization, but also from the plant side specific features are needed, such as plant genotype (cultivar) and developmental stage. Via multivariate analysis we present a quantification of the roles of these components on the composition of root-associated and endophytic bacterial communities in potato plants, by weighing the effects of bacterial inoculation, plant genotype and developmental stage. Spontaneous rifampicin resistant mutants of two bacterial endophytes, Paenibacillus sp. strain E119 and Methylobacterium mesophilicum strain SR1.6/6, were introduced into potato plants of three different cultivars (Eersteling, Robijn and Karnico). Densities of both strains in, or attached to potato plants were measured by selective plating, while the effects of bacterial inoculation, plant genotype and developmental stage on the composition of bacterial, Alphaproteobacterial and Paenibacillus species were determined by PCR-denaturing gradient gel-electrophoresis (DGGE). Multivariate analyses revealed that the composition of bacterial communities was mainly driven by cultivar type and plant developmental stage, while Alphaproteobacterial and Paenibacillus communities were mainly influenced by bacterial inoculation. These results are important for better understanding the effects of bacterial inoculations to plants and their possible effects on the indigenous bacterial communities in relation with other plant factors such as genotype and growth stage.

• Publisher: Dordrecht : Springer Netherlands,Springer Netherlands,Springer,Springer Nature B.V
• Subject: 16s ribosomal-rna / Bacteria / bacterial communities / Bacteriology / Biodiversity / Biological and medical sciences / Biomedical and Life Sciences / chemistry / classification / Colonization / Colony Count, Microbial / Cultivars / denaturing gradient gel electrophoresis / Developmental stages / diversity / DNA Fingerprinting / DNA, Bacterial / DNA, Bacterial - chemistry / DNA, Bacterial - genetics / Electrophoresis, Polyacrylamide Gel / Endophytes / Fundamental and applied biological sciences. Psychology / genetics / Genotype / Genotype & phenotype / Genotypes / gradient gel-electrophoresis / growth & development / Growth stage / Inoculation / land-use history / Life Sciences / Medical Microbiology / Methylobacterium / Methylobacterium - classification / Methylobacterium - growth & development / Methylobacterium - physiology / Methylobacterium mesophilicum / Microbiology / Miscellaneous / Molecular Sequence Data / multivariate analyses / Multivariate analysis / mutants / Nucleic Acid Denaturation / Original Paper / Paenibacillus / Paenibacillus - classification / Paenibacillus - growth & development / Paenibacillus - physiology / physiology / Plant communities / plant development / Plant Roots / Plant Roots - microbiology / Plant Sciences / Plant species / Potatoes / Resistant mutant / Rhizosphere / rifampicin / Rifampin / Roots / Sequence Analysis, DNA / soil / Soil microorganisms / Soil Science & Conservation / Solanum tuberosum / Solanum tuberosum - genetics / Solanum tuberosum - growth & development / Solanum tuberosum - microbiology / Symbiosis / transgenic eucalyptus / Vegetables / wild-type / xylella-fastidiosa