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Regulatory control of DNA end resection by Sae2 phosphorylation
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Regulatory control of DNA end resection by Sae2 phosphorylation
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Journal Article
Regulatory control of DNA end resection by Sae2 phosphorylation
2018
Overview
DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and are instead channeled to homology-directed repair. Using biochemical, genetic, and imaging methods, we show that phosphorylation of
Saccharomyces cerevisiae
Sae2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends by at least two distinct mechanisms. First, DNA damage and cell cycle-dependent phosphorylation leads to Sae2 tetramerization. Second, and independently, phosphorylation of the conserved C-terminal domain of Sae2 is a prerequisite for its physical interaction with Rad50, which is also crucial to promote the MRX endonuclease. The lack of this interaction explains the phenotype of
rad50S
mutants defective in the processing of Spo11-bound DNA ends during meiotic recombination. Our results define how phosphorylation controls the initiation of DNA end resection and therefore the choice between the key DNA double-strand break repair mechanisms.
It has previously been established that DNA end resection in yeast and in humans is under CDK control. Here the authors explain how phosphorylation regulates the capacity of Sae2 — the yeast orthologue of human CtIP — to promote DNA end resection.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/3
/ 42/70
/ 82/16
/ 82/29
/ 82/80
/ 82/83
/ DNA
/ DNA End-Joining Repair - physiology
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Endodeoxyribonucleases - metabolism
/ Exodeoxyribonucleases - metabolism
/ Homology
/ Humanities and Social Sciences
/ Meiosis
/ Multiprotein Complexes - chemistry
/ Multiprotein Complexes - metabolism
/ Nuclease
/ Recombinational DNA Repair - physiology
/ Repair
/ Saccharomyces cerevisiae - enzymology
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae - metabolism
/ Saccharomyces cerevisiae Proteins
/ Saccharomyces cerevisiae Proteins - chemistry
/ Saccharomyces cerevisiae Proteins - genetics
/ Saccharomyces cerevisiae Proteins - metabolism
/ Science
/ Yeast
