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Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae
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Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae
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Journal Article
Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae
2017
Overview
Background
Fungi are constantly exposed to nitrogen limiting environments, and thus the efficient regulation of nitrogen metabolism is essential for their survival, growth, development and pathogenicity. To understand how the rice blast pathogen
Magnaporthe oryzae
copes with limited nitrogen availability, a global proteome analysis under nitrogen supplemented and nitrogen starved conditions was completed.
Methods
M. oryzae
strain 70–15 was cultivated in liquid minimal media and transferred to media with nitrate or without a nitrogen source. Proteins were isolated and subjected to unfractionated gel-free based liquid chromatography-tandem mass spectrometry (LC-MS/MS). The subcellular localization and function of the identified proteins were predicted using bioinformatics tools.
Results
A total of 5498
M. oryzae
proteins were identified. Comparative analysis of protein expression showed 363 proteins and 266 proteins significantly induced or uniquely expressed under nitrogen starved or nitrogen supplemented conditions, respectively. A functional analysis of differentially expressed proteins revealed that during nitrogen starvation nitrogen catabolite repression, melanin biosynthesis, protein degradation and protein translation pathways underwent extensive alterations. In addition, nitrogen starvation induced accumulation of various extracellular proteins including small extracellular proteins consistent with observations of a link between nitrogen starvation and the development of pathogenicity in
M. oryzae
.
Conclusion
The results from this study provide a comprehensive understanding of fungal responses to nitrogen availability.
Publisher
BioMed Central,BioMed Central Ltd,BMC
