Journal Article

The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis

Zhang, Chun-Ling, Zhang, Ya-Li, Zhao, Qiang, Li, Yuan-Yuan, Wang, Yong-Xu, Qi, Chen-Hui, Hao, Yu-Jin, Wang, Gui-Luan, You, Chun-Xiang

2019

Overview

Background The MYB transcription factor family is one of the largest transcriptional factor families in plants and plays a multifaceted role in plant growth and development. However, MYB transcription factors involved in pathogen resistance in apple remain poorly understood. Results We identified a new MYB family member from apple, and named it MdMYB30. MdMYB30 was localized to the nucleus, and was highly expressed in young apple leaves. Transcription of MdMYB30 was induced by abiotic stressors, such as polyethylene glycol and abscisic acid. Scanning electron microscopy and gas chromatograph–mass spectrometry analyses demonstrated that ectopically expressing MdMYB30 in Arabidopsis changed the wax content, the number of wax crystals, and the transcription of wax-related genes. MdMYB30 bound to the MdKCS1 promoter to activate its expression and regulate wax biosynthesis. MdMYB30 also contributed to plant surface properties and increased resistance to the bacterial strain Pst DC3000. Furthermore, a virus-based transformation in apple fruits and transgenic apple calli demonstrated that MdMYB30 increased resistance to Botryosphaeria dothidea . Our findings suggest that MdMYB30 plays a vital role in the accumulation of cuticular wax and enhances disease resistance in apple. Conclusions MdMYB30 bound to the MdKCS1 gene promoter to activate its transcription and regulate cuticular wax content and composition, which influenced the surface properties and expression of pathogenesis-related genes to resistance against pathogens. MdMYB30 appears to be a crucial element in the formation of the plant cuticle and confers apple with a tolerance to pathogens.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: abiotic stress / Abscisic acid / Agriculture / Amino Acid Sequence / Apple / Apples / Arabidopsis / Arabidopsis - genetics / Arabidopsis - physiology / Arabidopsis thaliana / Ascomycota - physiology / Biomedical and Life Sciences / biosynthesis / Botryosphaeria dothidea / callus / Cuticular wax / Disease Resistance / DNA binding proteins / Ectopic Gene Expression / Electron microscopy / epicuticular wax / family / Fruits (Food) / Gene expression / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genetic engineering / genetically modified organisms / Glycols (Class of compounds) / growth and development / Host-Pathogen Interactions / Life Sciences / Malus - genetics / Malus - metabolism / Malus - microbiology / Mass spectrometry / MdMYB30 / Microbial drug resistance / Microscopy / Pathogenic microorganisms / pathogens / Pathogens resistance / plant cuticle / Plant Diseases - genetics / Plant Diseases - microbiology / Plant Epidermis - genetics / plant growth / Plant hormones / Plant Proteins - chemistry / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Plant-biotic interactions / Plants, Genetically Modified - genetics / Plants, Genetically Modified - physiology / Polyethylene glycol / Polyols / Research Article / RNA, Plant - analysis / Sequence Alignment / Spectroscopy / Surface science / Transcription factors / Transcription Factors - chemistry / Transcription Factors - genetics / Transcription Factors - metabolism / Tree Biology / Waxes - metabolism