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ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling
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ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling
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Journal Article
ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling
2021
Overview
Lung adenocarcinoma (LAC) is the most prevalent form of lung cancer. Epithelial cell transforming sequence 2 (ECT2) is a guanine nucleotide exchange factor that has been implicated in oncogenic and malignant phenotypes of LAC. Here, we identified an oncogenic role of ECT2 in the extracellular matrix (ECM) dynamics of LAC cells. We showed that suppression of ECT2 decreased adhesion and spreading of LAC cells on ECM components. Morphologically, ECT2‐depleted cells exhibited a rounded shape and cytoskeletal changes. Examination of transcriptional changes by RNA sequencing revealed a total of 1569 and 828 genes whose expressions were altered (absolute fold change and a difference of >2 fold) in response to suppression of ECT2 in two LAC cells (Calu‐3 and NCI‐H2342), respectively, along with 298 genes that were common to both cell lines. Functional enrichment analysis of common genes demonstrated a significant enrichment of focal adhesions. In accord with this observation, we found that ECT2 suppression decreased the expression level of proteins involved in focal adhesion signaling including focal adhesion kinase (FAK), Crk, integrin β1, paxillin, and p130Cas. FAK knockdown leads to impaired cell proliferation, adhesion, and spreading of LAC cells. Moreover, in LAC cells, ECT2 binds to and stabilizes FAK and is associated with the formation of the focal adhesions. Our findings provide new insights into the underlying role of ECT2 in cell‐ECM dynamics during LAC progression and suggest that ECT2 could be a promising therapeutic avenue for lung cancer. In this study, we demonstrated that epithelial cell transforming sequence 2 (ECT2) is an essential mediator of cell‐extracellular matrix (ECM) dynamics in the malignant progression of lung adenocarcinoma. We found that suppression of ECT2 decreased adhesion and spreading of lung adenocarcinoma cells on the ECM and demonstrated that ECT2 has a regulatory role in focal adhesion signaling, being associated with the focal adhesion formation that contains both focal adhesion kinase (FAK) and ECT2.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc
Subject
/ Adenocarcinoma of Lung - metabolism
/ Adenocarcinoma of Lung - pathology
/ Cell Proliferation - physiology
/ DNA
/ ECT2
/ Extracellular Matrix - metabolism
/ Extracellular Matrix - pathology
/ Focal Adhesion Protein-Tyrosine Kinases - metabolism
/ Focal Adhesions - metabolism
/ Gene Expression Regulation, Neoplastic - physiology
/ Gene set enrichment analysis
/ Guanine
/ Guanine nucleotide exchange factor
/ Humans
/ Kinases
/ Original
/ Paxillin
/ Proteins
/ Proto-Oncogene Proteins - metabolism
/ RNA
