Journal Article

A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity

Hitch, Thomas C. A., Wylensek, David, Hernández, Sara B., Gulder, Tobias A. M., Lagkouvardos, Ilias, Lesker, Till R., Afrizal, Afrizal, M. D’Agostino, Paul, Suarez, Laura Navarro, Allen-Vercoe, Emma, Liu, Tianzhe, de Wouters, Tomas, Reichlin, Markus, Segata, Nicola, Abt, Birte, Neumann, Anthony P., Neuhaus, Klaus, Wyschkon, Marzena, Doden, Heidi L., Strowig, Till, Taverne-Thiele, Anja J., Clavel, Thomas, Flisikowski, Krzysztof, Elling, Lothar, Fischöder, Thomas, Giesbers, Marcel, Kumar, Neeraj, Ridlon, Jason M., Cumbo, Fabio, Alves, João M. P., Suen, Garret, Schnieke, Angelika, Bunk, Boyke, Wortmann, Esther, Spröer, Cathrin, Buhl, Eva M., Cava, Felipe, Looft, Torey, Ly, Lindsey, Parreira, Valeria R., Rohn, Sascha, Devendran, Saravanan, Riedel, Thomas, Wells, Jerry M., Overmann, Jörg, Heine, Viktoria

2020

Overview

Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus , Streptococcus , Clostridium , Desulfovibrio , Enterococcus , Fusobacterium , and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci , and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae . The strain collection, called ‘Pig intestinal bacterial collection’ (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota. The authors present a public collection of 117 bacterial isolates from the pig gut, including the description of 38 novel taxa. Interesting functions discovered in these organisms include a new fucosyltransferease and sactipeptide-like molecules encoded by biosynthetic gene clusters.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 101/28 / 140/58 / 38/47 / 45/43 / 49/22 / 49/23 / 631/114/739 / 631/326/2565/2134 / 631/326/2565/2142 / 631/326/41/2530 / 631/61/212 / 82/81 / Aged, 80 and over / Agricultural research / Animals / Bacteria / Bacteria - classification / Bacteria - genetics / Bacteria - isolation & purification / Bacteria - metabolism / Bile / Bile acids / Bile Acids and Salts - metabolism / Biodiversity / Biosynthesis / Cell culture / Clostridium / Clostridium - classification / Clostridium - genetics / Clostridium - isolation & purification / Collection / Digestive system / Feces - microbiology / Female / Gastrointestinal Microbiome - genetics / Gastrointestinal tract / Gene clusters / Genes, Bacterial - genetics / Genomes / Host Specificity / Humanities and Social Sciences / Humans / Intestinal microflora / Intestine / Intestines - microbiology / Male / Metagenome / Microbiota / multidisciplinary / Multigene Family / New genera / Peptides / Phylogeny / RNA, Ribosomal, 16S / rRNA 16S / Science / Science (multidisciplinary) / Species / Swine / Swine - microbiology / Taxonomy