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Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3
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Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3
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Journal Article
Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3
2000
Overview
We present evidence that both corepressors SMRT and N‐CoR exist in large protein complexes with estimated sizes of 1.5–2 MDa in HeLa nuclear extracts. Using a combination of conventional and immunoaffinity chromatography, we have successfully isolated a SMRT complex and identified histone deacetylase 3 (HDAC3) and transducin (β)‐like I (TBL1), a WD‐40 repeat‐containing protein, as the subunits of the purified SMRT complex. We show that the HDAC3‐containing SMRT and N‐CoR complexes can bind to unliganded thyroid hormone receptors (TRs)
in vitro
. We demonstrate further that in
Xenopus
oocytes, both SMRT and N‐CoR also associate with HDAC3 in large protein complexes and that injection of antibodies against HDAC3 or SMRT/N‐CoR led to a partial relief of repression by unliganded TR/RXR. These findings thus establish both SMRT and N‐CoR complexes as bona fide HDAC‐containing complexes and shed new light on the molecular pathways by which N‐CoR and SMRT function in transcriptional repression.
Publisher
John Wiley & Sons, Ltd,Nature Publishing Group UK,Springer Nature B.V,Oxford University Press
Subject
/ Chromatography, Ion Exchange
/ DNA-Binding Proteins - chemistry
/ DNA-Binding Proteins - isolation & purification
/ DNA-Binding Proteins - metabolism
/ HDAC3
/ Histone Deacetylases - chemistry
/ Histone Deacetylases - metabolism
/ Humans
/ Ligands
/ Nuclear Proteins - chemistry
/ Nuclear Proteins - metabolism
/ Nuclear Receptor Co-Repressor 1
/ Nuclear Receptor Co-Repressor 2
/ Receptors, Retinoic Acid - metabolism
/ Receptors, Thyroid Hormone - metabolism
/ Recombinant Proteins - metabolism
/ Repressor Proteins - chemistry
/ Repressor Proteins - genetics
/ Repressor Proteins - isolation & purification
/ Repressor Proteins - metabolism
/ SMRT and N-CoR corepressor complexes
/ TBL1
/ Thyroid
/ Two-Hybrid System Techniques
/ Xenopus
