Journal Article

A Network Pharmacology-Based Strategy For Predicting Active Ingredients And Potential Targets Of LiuWei DiHuang Pill In Treating Type 2 Diabetes Mellitus

Du, Qing, Qin, Yu-hui, Zhang, Si-fang, Zhang, Zhe-yu, He, Dan, Yu, Rong, Zhang, Shui-han, Peng, Wei-jun, Huang, Jian-hua

2019

Overview

Traditional Chinese medicine (TCM) formulations have proven to be advantageous in clinical treatment and prevention of disease. LiuWei DiHuang Pill (LWDH Pill) is a TCM that was employed to treat type 2 diabetes mellitus (T2DM). However, a holistic network pharmacology approach to understanding the active ingredients and the therapeutic mechanisms underlying T2DM has not been pursued. A network pharmacology approach including drug-likeness evaluation, oral bioavailability prediction, virtual docking, and network analysis has been used to predict the active ingredients and potential targets of LWDH Pill in the treatment of type 2 diabetes. The comprehensive network pharmacology approach was successfully to identify 45 active ingredients in LWDH Pill. 45 active ingredients hit by 163 potential targets related to T2DM. Ten of the more highly predictive components (such as :quercetin, Kaempferol, Stigmasterol, beta-sitosterol, Kadsurenone, Diosgenin, hancinone C, Hederagenin, Garcinone B, Isofucosterol) are involved in anti-inflammatory, anti-oxidative stress, and the reduction of beta cell damage. LWDH Pill may play a role in the treatment of T2DM and its complications (atherosclerosis and nephropathy) through the AGE-RAGE signaling pathway, TNF signaling pathway, and NF-kappa B signaling pathway. Based on a systematic network pharmacology approach, our works successfully predict the active ingredients and potential targets of LWDH Pill for application to T2DM and helps to illustrate mechanism of action on a comprehensive level. This study provides identify key genes and pathway associated with the prognosis and pathogenesis of T2DM from new insights, which also demonstrates a feasible method for the research of chemical basis and pharmacology in LWDH Pill.

• Publisher: Dove Medical Press Limited,Taylor & Francis Ltd,Dove Press,Dove,Dove Medical Press
• Subject: Alzheimer's disease / Arteriosclerosis / Asian medicine / Atherosclerosis / Beta cells / Bioavailability / Cluster Analysis / Complications / Cost estimates / Crystal structure / Diabetes / Diabetes mellitus / Diabetes mellitus (non-insulin dependent) / Diabetes Mellitus, Type 2 - drug therapy / Drug therapy / Drugs, Chinese Herbal - chemistry / Drugs, Chinese Herbal - isolation & purification / Drugs, Chinese Herbal - therapeutic use / Formulations / Genes / Health aspects / Herbal medicine / Humans / Hypoglycemic Agents - chemistry / Hypoglycemic Agents - isolation & purification / Hypoglycemic Agents - therapeutic use / Inflammation / Ingredients / Kaempferol / Kidney diseases / Ligands / LWDH Pill / Medical treatment / Nephropathy / Network analysis / network pharmacology / network pharmacologyCorrigendum for this paper has been published / NF-κB protein / Organic chemistry / Original Research / Oxidative stress / Pathogenesis / Pharmacology / Phytosterols / Predictions / Progesterone / Proteins / Quercetin / Signal transduction / Signaling / Sterols / T2DM / Traditional Chinese medicine / Tumor necrosis factor / Type 2 diabetes / type 2 diabetes mellitus / type 2 diabetes mellitus (t2dm)