Journal Article

Tissue-based map of the human proteome

Berling, Holger, von Feilitzen, Kalle, Sjöstedt, Evelina, Schwenk, Jochen M., Lundberg, Emma, Navani, Sanjay, Uhlén, Mathias, Hober, Sophia, Edlund, Karolina, Hallström, Björn M., Pontén, Fredrik, Fagerberg, Linn, Nielsen, Jens, Olsson, IngMarie, Rockberg, Johan, Tegel, Hanna, Sivertsson, Åsa, Takanen, Jenny Ottosson, Asplund, Anna, Mardinoglu, Adil, Edqvist, Per-Henrik, Forsberg, Mattias, Lindskog, Cecilia, von Heijne, Gunnar, Alm, Tove, Nilsson, Peter, Djureinovic, Dijana, Kampf, Caroline, Persson, Lukas, Oksvold, Per, Zwahlen, Martin, Johansson, Fredric, Szigyarto, Cristina Al-Khalili, Mulder, Jan, Hamsten, Marica, Odeberg, Jacob

2015

Overview

Sequencing the human genome gave new insights into human biology and disease. However, the ultimate goal is to understand the dynamic expression of each of the approximately 20,000 protein-coding genes and the function of each protein. Uhlén et al. now present a map of protein expression across 32 human tissues. They not only measured expression at an RNA level, but also used antibody profiling to precisely localize the corresponding proteins. An interactive website allows exploration of expression patterns across the human body. Science , this issue 10.1126/science.1260419 Transcriptomics and immunohistochemistry map protein expression across 32 human tissues. Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray–based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.

• Publisher: American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Adipose tissue / Biology / Coding / Gene expression / Genes / Human Body / Human tissues / Interactive / isoprotein / membrane protein / Membranes / Metabolism / Narcotics / Organs / protein / Protein expression / Proteins / proteome / Proteomics / RESEARCH ARTICLE SUMMARY / tumor protein