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Regulation of chemotropic guidance of nerve growth cones by microRNA
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Regulation of chemotropic guidance of nerve growth cones by microRNA
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Journal Article
Regulation of chemotropic guidance of nerve growth cones by microRNA
2011
Overview
Background
The small non-coding microRNAs play an important role in development by regulating protein translation, but their involvement in axon guidance is unknown. Here, we investigated the role of microRNA-134 (miR-134) in chemotropic guidance of nerve growth cones.
Results
We found that miR-134 is highly expressed in the neural tube of
Xenopus
embryos. Fluorescent in situ hybridization also showed that miR-134 is enriched in the growth cones of
Xenopus
spinal neurons in culture. Importantly, overexpression of miR-134 mimics or antisense inhibitors blocked protein synthesis (PS)-dependent attractive responses of
Xenopus
growth cones to a gradient of brain-derived neurotrophic factor (BDNF). However, miR-134 mimics or inhibitors had no effect on PS-independent bidirectional responses of
Xenopus
growth cones to bone morphogenic protein 7 (BMP7). Our data further showed that
Xenopus
LIM kinase 1 (Xlimk1) mRNA is a potential target of miR-134 regulation.
Conclusions
These findings demonstrate a role for miR-134 in translation-dependent guidance of nerve growth cones. Different guidance cues may act through distinct signaling pathways to elicit PS-dependent and -independent mechanisms to steer growth cones in response to a wide array of spatiotemporal cues during development.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ BDNF
/ Biomedical and Life Sciences
/ BMP7
/ Brain
/ Brain-derived neurotrophic factor
/ Brain-Derived Neurotrophic Factor - pharmacology
/ Cloning
/ Embryos
/ Fluorescent Antibody Technique
/ Gene Expression Regulation - drug effects
/ Humans
/ MicroRNA
/ Mitogen-Activated Protein Kinase 1 - metabolism
/ Neurons
/ Oligonucleotides, Antisense - pharmacology
/ Phosphorylation - drug effects
/ Xenopus
