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Sphingosine-1-Phosphate Regulates the Expression of Adherens Junction Protein E-Cadherin and Enhances Intestinal Epithelial Cell Barrier Function
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Sphingosine-1-Phosphate Regulates the Expression of Adherens Junction Protein E-Cadherin and Enhances Intestinal Epithelial Cell Barrier Function
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Journal Article
Sphingosine-1-Phosphate Regulates the Expression of Adherens Junction Protein E-Cadherin and Enhances Intestinal Epithelial Cell Barrier Function
2011
Overview
Background
The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions.
Methods
Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques.
Results
S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell–cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER)
in vitro
.
Conclusions
These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell–cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells.
Publisher
Springer US,Springer,Springer Nature B.V
Subject
Adherens Junctions - metabolism
/ Animals
/ Biological and medical sciences
/ Cell Adhesion Molecules - genetics
/ Cell Adhesion Molecules - metabolism
/ Epithelial Cells - drug effects
/ Epithelial Cells - physiology
/ Ethylenediaminetetraacetic acid
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation - drug effects
/ Gene Expression Regulation - physiology
/ Intestinal Mucosa - cytology
/ Lysophospholipids - pharmacology
/ Medicine
/ Oncology
/ RNA
/ Sphingosine - analogs & derivatives
/ Vertebrates: anatomy and physiology, studies on body, several organs or systems
