Asset Details
Do you wish to reserve the book?
An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells
Do you wish to request the book?
An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
An immunofluorescence microscopy assay to discriminate distinct expression patterns of HIV-1 Gag and Nef proteins in HIV-1 provirus-harboring cells
2026
Overview
Over 95% of HIV-1 proviruses are defective and were once considered clinically irrelevant. However, growing evidence shows that these defective proviruses can still be transcribed and translated into viral proteins. Here, we developed an improved immunofluorescence protocol that combines two anti-Nef antibodies with one anti-Gag antibody, along with membrane and nuclear staining, enabling direct visualization of protein expression and localization. This method allows detailed characterization of the expression patterns and subcellular distribution of Gag and Nef proteins derived from defective proviruses. The protocol provides a practical tool for investigating the potential functions of proteins expressed from defective HIV-1 proviruses and for facilitating the ability to determine the biologic activity of cells harboring defective HIV-1 proviruses in patients living with HIV.
Publisher
Public Library of Science,PLOS
Subject
/ Cloning
/ Fluorescent antibody technique
/ gag Gene Products, Human Immunodeficiency Virus - genetics
/ gag Gene Products, Human Immunodeficiency Virus - metabolism
/ Genomes
/ HIV
/ Human immunodeficiency virus
/ Humans
/ Lectins
/ Medicine and Health Sciences
/ Microscopy, Fluorescence - methods
/ nef Gene Products, Human Immunodeficiency Virus - genetics
/ nef Gene Products, Human Immunodeficiency Virus - metabolism
/ Plasma
/ Proteins
/ Research and Analysis Methods
/ Viruses
