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Molecular and functional characterization of porcine poly C binding protein 1 (PCBP1)
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Molecular and functional characterization of porcine poly C binding protein 1 (PCBP1)
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Molecular and functional characterization of porcine poly C binding protein 1 (PCBP1)
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Journal Article
Molecular and functional characterization of porcine poly C binding protein 1 (PCBP1)
2024
Overview
Background
Poly C Binding Protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family. It is a multifunctional protein that participates in several functional circuits and plays a variety of roles in cellular processes. Although PCBP1 has been identified in several mammals, its function in porcine was unclear.
Results
In this study, we cloned the gene of porcine PCBP1 and analyzed its evolutionary relationships among different species. We found porcine PCBP1 protein sequence was similar to that of other animals. The subcellular localization of PCBP1 in porcine kidney cells 15 (PK-15) cells was analyzed by immunofluorescence assay (IFA) and revealed that PCBP1 was mainly localized to the nucleus. Reverse transcription-quantitative PCR (RT-qPCR) was used to compare PCBP1 mRNA levels in different tissues of 30-day-old pigs. Results indicated that PCBP1 was expressed in various tissues and was most abundant in the liver. Finally, the effects of PCBP1 on cell cycle and apoptosis were investigated following its overexpression or knockdown in PK-15 cells. The findings demonstrated that PCBP1 knockdown arrested cell cycle in G0/G1 phase, and enhanced cell apoptosis.
Conclusions
Porcine PCBP1 is a highly conserved protein, plays an important role in determining cell fate, and its functions need further study.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Analysis
/ Animals
/ Carrier Proteins - metabolism
/ Cloning
/ Diseases
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ family
/ fluorescent antibody technique
/ G1 phase
/ genes
/ Heterogeneous-Nuclear Ribonucleoproteins - genetics
/ Heterogeneous-Nuclear Ribonucleoproteins - metabolism
/ Kidneys
/ liver
/ Mammals
/ Medicine
/ Plasmids
/ Porcine poly C binding protein 1
/ Proteins
/ RNA-Binding Proteins - genetics
/ RNA-Binding Proteins - metabolism
/ species
/ Swine
/ Veterinary Medicine/Veterinary Science
/ Viruses
/ Zoology
