Journal Article

High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China

Chen, Weiwei, Zhan, Qing, Yu, Xiao, Teng, Gaoqin

2024

Overview

Objectives Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China. Methods From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6 hospitals in 4 cities in Zhejiang province, east China. Antimicrobial susceptibility tests were conducted according to the guidelines set forth by the Clinical and Laboratory Standards Institute (CLSI). To characterize and analyze these isolates, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCC mec ) typing, staphylococcal protein A ( spa ) typing and virulence genes gene profiles were performed. Results The most predominant clone was ST5-SCC mec II-t311, which accounted for 41.8% (110/263), followed by ST59 (44/263, 16.7%). Compared with non-ST5-II-t311 isolates, ST5-II-t311 isolates were more resistant to erythromycin, tetracycline, levofloxacin, moxifloxacin, and ciprofloxacin, but more susceptible to clindamycin. Moreover, the rates of multidrug resistance were higher in ST5-II-t311 isolates compared to the non-ST5-II-t311 isolates. In comparison to the non-ST5-II-t311 isolates, ST5-II-t311 isolates showed no significant difference in virulence genes detected. Conclusions MRSA ST5-II-t311 clone has become the most predominant clone in Zhejiang Province, east China and has higher rates of multidrug resistance than other isolates, that should be kept in mind when treating BSI. Moreover, MRSA ST59 clone shows an upward trend and has begun to spread into hospitals. Our findings highlight the importance of epidemiological studies of S. aureus carriage in the eastern region.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Antibiotics / Antimicrobial agents / Antimicrobial susceptibility / Bacterial infections / Biological Microscopy / Biomedical and Life Sciences / Bloodstream infections (BSIs) / Care and treatment / Chromosomes / Ciprofloxacin / Clindamycin / Cloning / Diagnosis / Disease susceptibility / Distribution / Drug resistance / Drug resistance in microorganisms / Epidemiology / Erythromycin / Genes / Global health / Health aspects / Health risks / Hospitals / Industrialized nations / Infection / Levofloxacin / Life Sciences / Medical colleges / Methicillin / Methicillin-resistant staphylococcus aureus (MRSA) / Microbiology / Morbidity / Mortality / Moxifloxacin / Multidrug resistance / Multilocus sequence typing / Mycology / Parasitology / Penicillin / Protein A / Public health / Sepsis / ST5-SCCmec II-t311 / Staphylococcus aureus / Staphylococcus aureus infections / Staphylococcus infections / Toxins / Virology / Virulence / Virulence (Microbiology) / Virulence genes / World health