Journal Article

FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155

Kjærup, Rikke Brødsgaard, Vervelde, Lonneke, Martinez, Fernando Garcia, Muñoz, Javier, Kolenda, Rafał, Kingsley, Robert A., Dalgaard, Tina Sørensen, Grzymajło, Krzysztof, Sives, Samantha, Borowska, Dominika, Aleksandrowicz, Adrianna

2024

Overview

Adaptation of avian pathogenic E. coli (APEC) to changing host environments including virulence factors expression is vital for disease progression. FdeC is an autotransporter adhesin that plays a role in uropathogenic Escherichia coli (UPEC) adhesion to epithelial cells. Expression of fdeC is known to be regulated by environmental conditions in UPEC and Shiga toxin-producing E. coli (STEC). The observation in a previous study that an APEC strain IMT5155 in which the fdeC gene was disrupted by a transposon insertion resulted in elevated adhesion to chicken intestinal cells prompted us to further explore the role of fdeC in infection. We found that the fdeC gene prevalence and FdeC variant prevalence differed between APEC and nonpathogenic E. coli genomes. Expression of the fdeC gene was induced at host body temperature, an infection relevant condition. Disruption of fdeC resulted in greater adhesion to CHIC-8E11 cells and increased motility at 42 °C compared to wild type (WT) and higher expression of multiple transporter proteins that increased inorganic ion export. Increased motility may be related to increased inorganic ion export since this resulted in downregulation of YbjN, a protein known to supress motility. Inactivation of fdeC in APEC strain IMT5155 resulted in a weaker immune response in chickens compared to WT in experimental infections. Our findings suggest that FdeC is upregulated in the host and contributes to interactions with the host by down-modulating motility during colonization. A thorough understanding of the regulation and function of FdeC could provide novel insights into E. coli pathogenesis.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: adhesin / adhesins / Adhesins, Escherichia coli - genetics / Adhesins, Escherichia coli - metabolism / adhesion / Animals / autotransporter / B cells / Bacterial Adhesion / birds / body temperature / Chickens / Development and progression / disease progression / epithelium / Escherichia coli / Escherichia coli - genetics / Escherichia coli - physiology / Escherichia coli Infections - microbiology / Escherichia coli Infections - veterinary / Escherichia coli Proteins - genetics / Escherichia coli Proteins - metabolism / Ethylenediaminetetraacetic acid / FdeC / Gene Expression Regulation, Bacterial / Genes / Genomics / Health aspects / immune response / Immunology / Infection / intestines / ion transport / Life Sciences / Medical research / Medicine / Medicine & Public Health / Medicine, Experimental / Microbiology / motility / pathogenesis / Poultry Diseases - microbiology / Proteins / Research Article / Shiga toxin-producing Escherichia coli / Transposons / uropathogenic Escherichia coli / Veterinary Medicine/Veterinary Science / Virology / virulence / Virulence (Microbiology)