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Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome
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Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome
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Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome
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Journal Article
Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome
2010
Overview
Background
Recent studies have suggested a role for an altered intestinal microbiota in the pathophysiology of irritable bowel syndrome (IBS). However, no consensus has been reached regarding the association between specific enteric bacterial groups and IBS. The aim of this study was to investigate the fecal and mucosal-associated microbiota using two independent techniques in intestinal samples from diarrhea-predominant IBS (D-IBS) and healthy controls.
Methods
Fecal and colonic mucosal biopsy samples were obtained from 10 D-IBS patients and 10 healthy controls. Colonic tissue was collected during a un-sedated un-prepped flexible sigmoidoscopy. Fecal and tissue samples were processed immediately upon collection for culture under aerobic and anaerobic conditions or frozen for further molecular analysis. DNA was extracted from all frozen samples and used to enumerate specific bacterial groups using quantitative real-time PCR (qPCR).
Results
Culture analysis of intestinal samples demonstrated a significant reduction in the concentration of aerobic bacteria in fecal samples from D-IBS patients when compared to healthy controls (1.4 × 10
7
vs. 8.4 × 10
8
CFUs/g feces,
P
= 0.002). qPCR analysis demonstrated a significant 3.6 fold increase (
P
= 0.02) in concentrations of fecal
Lactobacillus
species between D-IBS patients and healthy controls.
Conclusions
Our culture and molecular data indicate that quantitative differences exist in specific bacterial groups in the microbiota between D-IBS and healthy subjects.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
