Journal Article

Comparison of Tuber Proteomes of Potato Varieties, Landraces, and Genetically Modified Lines

Auriola, Seppo, Biogéochimie et écologie des milieux continentaux (Bioemco) ; École normale supérieure - Paris (ENS-PSL) ; Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), M. Koistinen, Kaisa, Suomalainen, Soile, V. Davies, Howard, Nunan, Naoise, V.T. Shepherd, Louise, O. Karenlampi, Sirpa, I. Kokko, Harri, W. Mcnicol, Jim, J. Lehesranta, Satu

2005

Overview

Crop improvement by genetic modification remains controversial, one of the major issues being the potential for unintended effects. Comparative safety assessment includes targeted analysis of key nutrients and antinutritional factors, but broader scale-profiling or \"omics\" methods could increase the chances of detecting unintended effects. Comparative assessment should consider the extent of natural variation and not simply compare genetically modified (GM) lines and parental controls. In this study, potato (Solanum tuberosum) proteome diversity has been assessed using a range of diverse non-GM germplasm. In addition, a selection of GM potato lines was compared to assess the potential for unintended differences in protein profiles. Clear qualitative and quantitative differences were found in the protein patterns of the varieties and landraces examined, with 1,077 of 1,111 protein spots analyzed showing statistically significant differences. The diploid species Solanum phureja could be clearly differentiated from tetraploid (Solanum tuberosum) genotypes. Many of the proteins apparently contributing to genotype differentiation are involved in disease and defense responses, the glycolytic pathway, and sugar metabolism or protein targeting/storage. Only nine proteins out of 730 showed significant differences between GM lines and their controls. There was much less variation between GM lines and their non-GM controls compared with that found between different varieties and landraces. A number of proteins were identified by mass spectrometry and added to a potato tuber two-dimensional protein map.

• Publisher: Oxford University Press ; American Society of Plant Biologists,HAL CCSD,American Society of Plant Biologists,American Society of Plant Physiologists
• Subject: Agronomy. Soil science and plant productions / amino acid sequences / Biological and medical sciences / chemical analysis / classification / diploidy / Ecology, environment / Economic plant physiology / Electrophoresis, Gel, Two-Dimensional / Energy metabolism / Fundamental and applied biological sciences. Psychology / Gels / genetic variation / genetics / Genetics, Genomics, and Molecular Evolution / Genotypes / Life Sciences / Metabolic diseases / metabolism / molecular sequence data / Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia) / Nutrition. Photosynthesis. Respiration. Metabolism / plant biochemistry / plant genetics / plant proteins / Plant Proteins - genetics / Plants / Plants, Genetically Modified / Plants, Genetically Modified - genetics / potatoes / Principal components analysis / Protein metabolism / Proteome / Proteomes / qualitative analysis / quantitative analysis / Recombinant Proteins / Recombinant Proteins - metabolism / Solanum phureja / Solanum tuberosum / Solanum tuberosum - classification / Solanum tuberosum - genetics / Solanum tuberosum subsp. andigenum / Transgenic plants / Tubers / two dimensional gel electrophoresis