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DRfit: a Java tool for the analysis of discrete data from multi-well plate assays

by Hofmann, Andreas , Simon, Anne , Cross, Megan , Gasser, Robin B. , Herath, H. M. P. Dilrukshi , Preston, Sarah

in Algorithms / Assaying / Biochemical assays / Bioinformatics / Biological Science Disciplines / Biomedical and Life Sciences / Bivariate analysis / Computational Biology/Bioinformatics / Computer Appl. in Life Sciences / Computer programs / Data acquisition / Data analysis / Data Interpretation, Statistical / Data points / Design / Dose-response experiments / Drug discovery / Enzyme assays / Enzymes / Graphical user interface / Java (Programming language) / Laboratories / Life Sciences / Methods / Microarrays / Multivariate analysis / Pharmaceutical research / Response functions / Results and data / Scientific software / Software / Software development tools / Technology application / User interface / User-Computer Interface

2019

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DRfit: a Java tool for the analysis of discrete data from multi-well plate assays

by Hofmann, Andreas , Simon, Anne , Cross, Megan , Gasser, Robin B. , Herath, H. M. P. Dilrukshi , Preston, Sarah

2019

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DRfit: a Java tool for the analysis of discrete data from multi-well plate assays

by Hofmann, Andreas , Simon, Anne , Cross, Megan , Gasser, Robin B. , Herath, H. M. P. Dilrukshi , Preston, Sarah

2019

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Journal Article

DRfit: a Java tool for the analysis of discrete data from multi-well plate assays

Hofmann, Andreas,

Simon, Anne,

Cross, Megan,

Gasser, Robin B.,

Herath, H. M. P. Dilrukshi,

Preston, Sarah

2019

Overview

Background Analyses of replicates in sets of discrete data, typically acquired in multi-well plate formats, is a recurring task in many contemporary areas in the Life Sciences. The availability of accessible cross-platform data analysis tools for such fundamental tasks in varied projects and environments is an important prerequisite to ensuring a reliable and timely turnaround as well as to provide practical analytical tools for student training. Results We have developed an easy-to-use, interactive software tool for the analysis of multiple data sets comprising replicates of discrete bivariate data points. For each dataset, the software identifies the replicate data points from a defined matrix layout and calculates their means and standard errors. The averaged values are then automatically fitted using either a linear or a logistic dose response function. Conclusions DRfit is a practical and convenient tool for the analysis of one or multiple sets of discrete data points acquired as replicates from multi-well plate assays. The design of the graphical user interface and the built-in analysis features make it a flexible and useful tool for a wide range of different assays.

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Publisher

BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC

Subject

/ Biological Science Disciplines

/ Biomedical and Life Sciences

/ Bivariate analysis