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Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus
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Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus
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Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus
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Journal Article
Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus
2020
Overview
Objective
This study aimed to evaluate the phenotypic and genotypic characterization of biofilm formation and
spa
and
ica
genes among clinical isolates of methicillin-resistant
Staphylococcus aureus
.
Result
This cross-sectional study was performed on 146
Staphylococcus aureus
isolates from hospitalized patients in Isfahan Province Hospitals. MRSA isolates were confirmed using disk diffusion test with oxacillin disk and amplification of
mecA
gene by PCR assays. Ability of biofilm production was evaluated targeting the
icaA and icaD
genes. Of 146
Staphylococcus aureus
isolates, 24 (16.4%) carried
mecA
genes and identified as MRSA strains. Strong ability of biofilm production was seen among 76.02% (111/146)
S. aureus
isolates and 87.5% (21/24) MRSA strains, respectively. Also, 75.0% (18/24) MRSA isolates carried
icaA
and
icaD
was not detected in these strains. Analysis of
spa
gene showed 70.83% (17/24) MRSA strains were
spa
positive. From which 14 and 3 strains identified with one band (150, 270, 300, 360, 400 bp) and two bands (150–300 bp), respectively. According to data obtained, the prevalence of MRSA isolates from Isfahan Province Hospitals is relatively high and a remarkable percentage of them show strong power in biofilm production. Also analysis of
spa
gene showed a fairly large diversity among MRSA strains.
