Journal Article

Comprehensive genomic characterization of NAC transcription factor family and their response to salt and drought stress in peanut

Li, Chunjuan, Sun, Quanxi, Shan, Shihua, Lu, Xiaodong, Yan, Caixia, Zhao, Xiaobo, Wang, Juan, Yuan, Cuiling

2020

Overview

Background Peanut is one of the most important oil crop species worldwide. NAC transcription factor (TF) genes play important roles in the salt and drought stress responses of plants by activating or repressing target gene expression. However, little is known about NAC genes in peanut. Results We performed a genome-wide characterization of NAC genes from the diploid wild peanut species Arachis duranensis and Arachis ipaensis , which included analyses of chromosomal locations, gene structures, conserved motifs, expression patterns, and cis -acting elements within their promoter regions. In total, 81 and 79 NAC genes were identified from A. duranensis and A. ipaensis genomes. Phylogenetic analysis of peanut NACs along with their Arabidopsis and rice counterparts categorized these proteins into 18 distinct subgroups. Fifty-one orthologous gene pairs were identified, and 46 orthologues were found to be highly syntenic on the chromosomes of both A. duranensis and A. ipaensis . Comparative RNA sequencing (RNA-seq)-based analysis revealed that the expression of 43 NAC genes was up- or downregulated under salt stress and under drought stress. Among these genes, the expression of 17 genes in cultivated peanut ( Arachis hypogaea ) was up- or downregulated under both stresses. Moreover, quantitative reverse transcription PCR (RT-qPCR)-based analysis revealed that the expression of most of the randomly selected NAC genes tended to be consistent with the comparative RNA-seq results. Conclusion Our results facilitated the functional characterization of peanut NAC genes, and the genes involved in salt and drought stress responses identified in this study could be potential genes for peanut improvement.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agriculture / Arabidopsis / Arabidopsis thaliana / Arachis - genetics / Arachis - physiology / Arachis duranensis / Arachis hypogaea / Arachis ipaensis / Biomedical and Life Sciences / Chromosome Mapping / Chromosomes / Chromosomes, Plant / Conserved Sequence / Diploids / diploidy / Drought / Droughts / Gene expression / Gene sequencing / Genes / Genes, Plant / Genetic aspects / Genetic research / Genome-wide characterization / Genomes / Genomics / Genomics and evolution / Legumes / Life Sciences / Multigene Family / NAC gene family / oil crops / Oilseeds / Peanut / Peanuts / Phylogenetics / Phylogeny / Plant Sciences / Proteins / Research Article / reverse transcriptase polymerase chain reaction / Reverse transcription / Ribonucleic acid / Rice / RNA / RNA sequencing / RNA-seq / RT-qPCR / Salt / Salt Stress / Salts / sequence analysis / Subgroups / Synteny / transcription factors / Transcription Factors - genetics / Transcription Factors - physiology / Tree Biology / water stress