Journal Article

Inflammatory mechanism of Rumenitis in dairy cows with subacute ruminal acidosis

Wang, Zhe, Liu, Guowen, Yuan, Xue, Sun, Guoquan, Wang, Yazhou, Li, Xiaobing, Zhao, Chenxu, Guan, Yuan, Li, Xinwei

2018

Overview

Background Subacute ruminal acidosis (SARA) is a metabolic disease in high-producing dairy cattle, and is accompanied by rumenitis. However, the mechanism of rumenitis remains unclear. Therefore, the aim of this study was to investigate the molecular mechanism of rumenitis in dairy cows with SARA. Results The results showed that SARA cows displayed high concentrations of ruminal volatile fatty acids, lactic acid and lipopolysaccharide (LPS). Furthermore, the blood concentrations of LPS and acute phase proteins haptoglobin, serum amyloid-A, and LPS binding protein were significantly higher in SARA cows than in control cows. Importantly, the phosphorylation levels of nuclear factor-kappaB (NF-κB) p65, inhibitor of NF-κB (IκB), c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase 1/2 (ERK1/2) were significantly higher in the rumen epithelium of SARA cows than those of control cows. The ruminal mRNA and protein levels of NF-κB- and mitogen-activated protein kinase (MAPK)s -regulated inflammatory cytokines, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL-1β), were markedly higher in SARA cows than in control cows. Similarly, serum concentrations of TNF-α and IL-6 were also significantly higher in SARA cows. Conclusions These results indicate that SARA results in high concentration of ruminal LPS, which over activates the NF-κB and MAPKs inflammatory pathways and then significantly increases the expression and synthesis of pro-inflammation cytokines in the rumen epithelium, thereby partly inducing rumenitis.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: acute phase proteins / binding proteins / blood serum / Clinical pathology / Dairy cattle / dairy cows / Genetic disorders / Gram-negative bacteria / haptoglobins / Health aspects / Inflammatory pathway / interleukin-1beta / interleukin-6 / lactic acid / Laminitis / lipopolysaccharides / LPS / Medicine / Medicine & Public Health / messenger RNA / mitogen-activated protein kinase / phosphorylation / physiology and immunology / Research Article / Risk factors / rumen epithelium / Rumenitis / ruminal acidosis / Subacute ruminal acidosis / transcription factor NF-kappa B / Transgenics / tumor necrosis factor-alpha / veterinary medicine / Veterinary Medicine/Veterinary Science / volatile fatty acids / Zoology