Journal Article

Single-cell transcriptome reveals dominant subgenome expression and transcriptional response to heat stress in Chinese cabbage

Liu, Mengyang, Ma, Wei, Zhang, Xiaomeng, Wang, Yanhua, Shen, Shuxing, Li, Yan, Sun, Xiaoxue, Feng, Daling, Lu, Yin, Qin, Ruixin, Zhao, Jianjun

2022

Overview

Background Chinese cabbage (Brassica rapa ssp. pekinensis) experienced a whole-genome triplication event and thus has three subgenomes: least fractioned, medium fractioned, and most fractioned subgenome. Environmental changes affect leaf development, which in turn influence the yield. To improve the yield and resistance to different climate scenarios, a comprehensive understanding of leaf development is required including insights into the full diversity of cell types and transcriptional networks underlying their specificity. Results Here, we generate the transcriptional landscape of Chinese cabbage leaf at single-cell resolution by performing single-cell RNA sequencing of 30,000 individual cells. We characterize seven major cell types with 19 transcriptionally distinct cell clusters based on the expression of the reported marker genes. We find that genes in the least fractioned subgenome are predominantly expressed compared with those in the medium and most fractioned subgenomes in different cell types. Moreover, we generate a single-cell transcriptional map of leaves in response to high temperature. We find that heat stress not only affects gene expression in a cell type-specific manner but also impacts subgenome dominance. Conclusions Our study highlights the transcriptional networks in different cell types and provides a better understanding of transcriptional regulation during leaf development and transcriptional response to heat stress in Chinese cabbage.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Animal Genetics and Genomics / Bioinformatics / Biomedical and Life Sciences / Brassica oleracea / Brassica rapa - genetics / Brassica rapa subsp. pekinensis / Chinese cabbage / Chloroplasts / climate / Climate change / Environmental changes / Evolutionary Biology / Gene expression / Gene Expression Regulation, Plant / Gene regulation / genome / Genomes / Heat / Heat resistance / Heat stress / Heat-Shock Response / High temperature / Human Genetics / Leaf development / Leaves / Life Sciences / Microbial Genetics and Genomics / Physiology / Plant Genetics and Genomics / Plant Proteins - genetics / RNA / Single-cell RNA sequencing / Subgenome dominance / temperature / Temperature effects / Transcription / transcription (genetics) / Transcriptome / Transcriptomes / Whole-genome triplication