Asset Details
Do you wish to reserve the book?
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection
Do you wish to request the book?
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection
2021
Overview
It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is developed for the sensitive protein detection and also can be used for the detection of pathogens. PLA employs aptamer or monoclonal/polyclonal antibody-nucleic acid complexes as proximity probes. When the paired proximity probes bind to the same target protein or protein complex, they will be adjacent to each other and form an amplifiable DNA sequence through ligation. Combining the specificity of enzyme-linked immunosorbent assay (ELISA) and sensitivity of polymerase chain reaction (PCR), PLA transforms the detection of protein into the detection of DNA nucleic acid sequence. Therefore, as an ultrasensitive protein assay, PLA has great potential for quantification, localization of protein, and clinical diagnostics. In this review, we summarize the basic principles of PLA and its applications in pathogen detection.Key Points• Different forms of proximity ligation assay are introduced.• Applications of proximity ligation assay in pathogen detection are summarized.• Proximity ligation assay is an ultrasensitive method to quantify protein and pathogen.
Publisher
Springer Nature B.V
