Journal Article

Graph construction method impacts variation representation and analyses in a bovine super-pangenome

Leonard, Alexander S., Mapel, Xena M., Bhati, Meenu, Pausch, Hubert, Crysnanto, Danang

2023

Overview

Background Several models and algorithms have been proposed to build pangenomes from multiple input assemblies, but their impact on variant representation, and consequently downstream analyses, is largely unknown. Results We create multi-species super-pangenomes using pggb, cactus, and minigraph with the Bos taurus taurus reference sequence and eleven haplotype-resolved assemblies from taurine and indicine cattle, bison, yak, and gaur. We recover 221 k nonredundant structural variations (SVs) from the pangenomes, of which 135 k (61%) are common to all three. SVs derived from assembly-based calling show high agreement with the consensus calls from the pangenomes (96%), but validate only a small proportion of variations private to each graph. Pggb and cactus, which also incorporate base-level variation, have approximately 95% exact matches with assembly-derived small variant calls, which significantly improves the edit rate when realigning assemblies compared to minigraph. We use the three pangenomes to investigate 9566 variable number tandem repeats (VNTRs), finding 63% have identical predicted repeat counts in the three graphs, while minigraph can over or underestimate the count given its approximate coordinate system. We examine a highly variable VNTR locus and show that repeat unit copy number impacts the expression of proximal genes and non-coding RNA. Conclusions Our findings indicate good consensus between the three pangenome methods but also show their individual strengths and weaknesses that need to be considered when analysing different types of variants from multiple input assemblies.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Animal Genetics and Genomics / Animals / Bioinformatics / Biomedical and Life Sciences / bison / Bos taurus taurus / Bovinae / Buffalo / cacti and succulents / Cattle / Cattle - genetics / Consortia / Copy number / Decomposition / Domestic animals / Evolutionary Biology / Genome / Genome assembly / Genomes / Graph pangenome / Graph representations / Haplotypes / Human Genetics / Life Sciences / loci / Long sequencing reads / Microbial Genetics and Genomics / Minisatellite Repeats / mithuns / non-coding RNA / Phylogenetics / Plant Genetics and Genomics / Sequence Analysis, DNA - methods / Structural variation / taurine / Variation / yaks