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Identification of an IRF8 gene in common carp (Cyprinus carpio. L) and its regulatory role in immune responses
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Identification of an IRF8 gene in common carp (Cyprinus carpio. L) and its regulatory role in immune responses
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Identification of an IRF8 gene in common carp (Cyprinus carpio. L) and its regulatory role in immune responses
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Journal Article
Identification of an IRF8 gene in common carp (Cyprinus carpio. L) and its regulatory role in immune responses
2025
Overview
Background
Interferon (IFN) regulatory factors (IRF) are the crucial transcription factors for IFN expression and leading host cells response to viral infection. IRF8 in mammals plays vital roles in the innate and adaptive immune systems. In this study, we identified and characterized the common carp (
Cyprinus carpio
. L)
IRF8
gene (cc
IRF8
) to further clarify the function of IRF8 in teleost fish.
Results
The complete cDNA sequence of cc
IRF8
was 1431 bp and encodes a polypeptide of 431 amino acids. Analysis of the putative amino acid sequence showed that ccIRF8 encodes structures typical of the IRF family, including a DNA-binding domain (DBD), an IRF-association domain (IAD) and two nuclear localization signals (NLS). Comparison with homologous proteins showed that the deduced protein has the highest sequence identity to grass carp IRF8 (92.7%). Phylogenetic analysis grouped ccIRF8 with other IRF8s of teleosts. Quantitative RT-PCR analysis showed that ccIRF8 transcripts were detectable in all investigated tissues of healthy fish with the highest level in spleen. Following poly I: C and
Aeromonas hydrophila
challenge, cc
IRF8
transcripts were induced significantly in immune relevant tissues. In addition, cc
IRF8
was induced by poly I: C and ipopolysaccharide (LPS), peptidoglycan (PGN) and flagellin in HKLs. Overexpression of cc
IRF8
increased the expression of
IFN
and IFN-stimulated genes (ISGs), and a dual-luciferase reporter assay revealed that cc
IRF8
decreased the activation of
NF-κB
though
TRAF6
.
Conclusions
Overall, our findings provide a new perspective on the role of IRF8 in innate immunity in fish, as well as insights that will help the prevention and control of disease in the common carp farming industry.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
Advancements in Veterinary Medicine and Aquaculture
/ Animals
/ Carp
/ Common carp (Cyprinus carpio. L)
/ domain
/ family
/ Fish
/ Gene Expression Regulation - immunology
/ Genes
/ Identification and classification
/ IFN
/ industry
/ Interferon Regulatory Factors - chemistry
/ Interferon Regulatory Factors - genetics
/ Interferon Regulatory Factors - immunology
/ Interferon Regulatory Factors - metabolism
/ IRF8
/ Medicine
/ NF-κB
/ Poly I:C
/ Proteins
/ reverse transcriptase polymerase chain reaction
/ Sequence Alignment - veterinary
/ spleen
/ Veterinary Medicine/Veterinary Science
/ Zoology
