Asset Details

MbrlCatalogueTitleDetail

Do you wish to reserve the book?

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

by Mizokami, Masashi , Date, Tomoko , Zhao, Zijiang , Liang, T Jake , Kräusslich, Hans-Georg , Bartenschlager, Ralf , Wakita, Takaji , Kato, Takanobu , Miyamoto, Michiko , Habermann, Anja , Murthy, Krishna , Pietschmann, Thomas

in Animals / Antibodies - pharmacology / Antigens, CD - immunology / Biomedical and Life Sciences / Biomedicine / Biophysical Phenomena / Biophysics / Cancer Research / Carcinoma, Hepatocellular - virology / Cell Line, Tumor / Cloning / Cloning, Molecular / Genome, Viral / Genomes / Hepacivirus - growth & development / Hepacivirus - isolation & purification / Hepacivirus - pathogenicity / Hepatitis / Hepatitis C / Hepatitis C - drug therapy / Hepatitis C - immunology / Hepatitis C virus / Humans / Immune Sera / Infectious Diseases / Life cycles / Liver Neoplasms - virology / Metabolic Diseases / Microscopy, Electron / Molecular Medicine / Monkeys & apes / Neurosciences / Pan troglodytes / Proteins / Public health / RNA polymerase / RNA, Viral / technical-report / Tetraspanin 28 / Transfection / Virology / Virus Cultivation - methods

2005

Yes Please

Hey, we have placed the reservation for you!

By the way, why not check out events that you can attend while you pick your title.

Oops! Something went wrong.

Looks like we were not able to place the reservation. Kindly try again later.

Are you sure you want to remove the book from the shelf?

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

2005

Confirm

Do you wish to request the book?

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

2005

Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy

How would you like to get it?

Submit

We have requested the book for you!

Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.

Oops! Something went wrong.

Looks like we were not able to place your request. Kindly try again later.

Journal Article

Production of infectious hepatitis C virus in tissue culture from a cloned viral genome

Mizokami, Masashi,

Date, Tomoko,

Zhao, Zijiang,

Liang, T Jake,

Kräusslich, Hans-Georg,

Bartenschlager, Ralf,

Wakita, Takaji,

Kato, Takanobu,

Miyamoto, Michiko,

Habermann, Anja,

Murthy, Krishna,

Pietschmann, Thomas

2005

Overview

Hepatitis C virus (HCV) infection causes chronic liver diseases and is a global public health problem. Detailed analyses of HCV have been hampered by the lack of viral culture systems. Subgenomic replicons of the JFH1 genotype 2a strain cloned from an individual with fulminant hepatitis replicate efficiently in cell culture. Here we show that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7). Particles have a density of about 1.15–1.17 g/ml and a spherical morphology with an average diameter of about 55 nm. Secreted virus is infectious for Huh7 cells and infectivity can be neutralized by CD81-specific antibodies and by immunoglobulins from chronically infected individuals. The cell culture–generated HCV is infectious for chimpanzee. This system provides a powerful tool for studying the viral life cycle and developing antiviral strategies.

Share this book

Add to My Shelf

Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

Animals

/ Antibodies - pharmacology

/ Antigens, CD - immunology

/ Biomedical and Life Sciences

/ Biomedicine

/ Biophysical Phenomena

/ Biophysics