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Super-resolution 3D tomography of interactions and competition in the nuclear pore complex
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Super-resolution 3D tomography of interactions and competition in the nuclear pore complex
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Journal Article
Super-resolution 3D tomography of interactions and competition in the nuclear pore complex
2016
Overview
Mapping the spatial distribution of interaction sites between FG nucleoporins and nuclear transport receptors within the native NPC through SPEED microscopy reveals the 3D configuration of the FG-nucleoporin barrier and competition among transport receptors.
A selective barrier formed by intrinsically disordered Phe-Gly (FG) nucleoporins (Nups) allows transport receptor (TR)-facilitated translocation of signal-dependent cargos through the nuclear pore complexes (NPCs) of eukaryotic cells. However, the configuration of the FG-Nup barrier and its interactions with multiple TRs in native NPCs remain obscure. Here, we mapped the interaction sites of various TRs or FG segments within the FG-Nup barrier by using high-speed super-resolution microscopy and used these sites to reconstruct the three-dimensional tomography of the native barrier in the NPC. We found that each TR possesses a unique interaction zone within the FG-Nup barrier and that two major TRs, importin β1 and Crm1, outcompete other TRs in binding FG Nups. Moreover, TRs may alter the tomography of the FG-Nup barrier and affect one another's pathways under circumstances of heavy competition.
Publisher
Nature Publishing Group US,Nature Publishing Group
