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A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis
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A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis
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Journal Article
A European inter-laboratory trial to evaluate the performance of three serological methods for diagnosis of Mycoplasma bovis infection in cattle using latent class analysis
2019
Overview
Background
Mycoplasma bovis
(
M. bovis
) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result in a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none of which are
M. bovis
-specific. Laboratory diagnosis is therefore important. Serological tests to detect
M. bovis
antibodies is considered an effective indicator of infection in a herd and often used as a herd test. Combined with clinical judgement, it can also be used to implement control strategies and/or to estimate the disease prevalence within a country. However, due to lack of harmonisation of approaches to testing, and serological tests used by different laboratories, comparisons of prevalence data between countries is often difficult. A network of researchers from six European countries designed and participated in an inter-laboratory trial, with the aim of evaluating the sensitivity (
Se
) and specificity
(Sp)
of two commercially available ELISA tests (ID Screen® ELISA (IDvet) and BIO K302 ELISA (BIO-X Diagnostics)) for diagnosis of
M. bovis
infection. Each laboratory received a blinded panel of bovine sera and tested independently, according to manufacturer’s instructions. Western blot analyses (WB) performed by one of the participating laboratories was used as a third diagnostic test in the statistical evaluation of
Se
and
Sp
values using latent class analysis.
Results
The
Se
of WB, the ID Screen® ELISA and the BIO K302 ELISA were determined to be 91.8, 93.5 and 49.1% respectively, and corresponding
Sp
of the three tests were 99.6, 98.6 and 89.6%, respectively.
Conclusions
The present study is, to our knowledge, the first to present an inter-laboratory comparison of the BIO K302 ELISA and the ID Screen® ELISA. Based on our results, the ID Screen® ELISA showed high consistency with WB and performed with higher precision and accuracy than the BIO K302 ELISA.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Blotting, Western - veterinary
/ Cattle
/ ELISA
/ Enzyme-linked immunosorbent assay
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - veterinary
/ Europe
/ herds
/ Mastitis
/ Medicine
/ Microbiology and Parasitology
/ Mycoplasma bovis - immunology
/ Mycoplasma Infections - blood
/ Mycoplasma Infections - diagnosis
/ Mycoplasma Infections - veterinary
/ Serologic Tests - veterinary
/ Veterinary Medicine/Veterinary Science
/ Zoology
